Anti-FACL4 antibody (ab227256)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-FACL4 antibody
See all FACL4 primary antibodies -
Description
Rabbit polyclonal to FACL4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human FACL4 (internal sequence). The exact sequence is proprietary.
Database link: O60488 -
Positive control
- WB: HeLa, RAW 264.7, PC-12, MCF7, MDA-MB-231 and Rat2 whole cell extracts. IHC-P: Mouse kidney tissue; Rat duodenum tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 79 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. -
Involvement in disease
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2182 Human
- Entrez Gene: 50790 Mouse
- Entrez Gene: 448980 Pig
- Entrez Gene: 113976 Rat
- Omim: 300157 Human
- SwissProt: O60488 Human
- SwissProt: Q9QUJ7 Mouse
- SwissProt: O35547 Rat
see all -
Alternative names
- ACS 4 antibody
- ACS4 antibody
- ACSL 4 antibody
see all
Images
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Western blot - Anti-FACL4 antibody (ab227256)All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : FACL4 shRNA-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (ab227256)Paraffin-embedded mouse kidney tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody (ab227256)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FACL4 (green) using ab227256 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (ab227256)Paraffin-embedded rat duodenum tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-FACL4 antibody (ab227256)Anti-FACL4 antibody (ab227256) at 1/1000 dilution + RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-FACL4 antibody (ab227256)All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-FACL4 antibody (ab227256)All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-FACL4 antibody (ab227256)Anti-FACL4 antibody (ab227256) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
References
ab227256 has not yet been referenced specifically in any publications.
