Overview

  • Product name

  • Description

    Rabbit polyclonal to FACL4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human FACL4 (internal sequence). The exact sequence is proprietary.
    Database link: O60488

  • Positive control

    • WB: HeLa, RAW 264.7, PC-12, MCF7, MDA-MB-231 and Rat2 whole cell extracts. IHC-P: Mouse kidney tissue; Rat duodenum tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 79 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
  • Involvement in disease

    Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACS 4 antibody
    • ACS4 antibody
    • ACSL 4 antibody
    • Acsl4 antibody
    • ACSL4_HUMAN antibody
    • acyl CoA synthetase 4 antibody
    • Acyl CoA synthetase long chain family member 4 antibody
    • FACL 4 antibody
    • FACL4 antibody
    • Fatty acid Coenzyme A ligase antibody
    • fatty acid Coenzyme A ligase long-chain 4 antibody
    • LACS 4 antibody
    • LACS4 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain 4 antibody
    • long chain acyl CoA synthetase 4 antibody
    • long chain fatty acid CoA ligase 4 antibody
    • long chain fatty acid Coenzyme A ligase 4 antibody
    • Long-chain acyl-CoA synthetase 4 antibody
    • Long-chain-fatty-acid--CoA ligase 4 antibody
    • MRX63 antibody
    • MRX68 antibody
    see all

Images

  • All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution

    Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 2 : FACL4 shRNA-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse kidney tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FACL4 (green) using ab227256 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).

  • Paraffin-embedded rat duodenum tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.

  • Anti-FACL4 antibody (ab227256) at 1/1000 dilution + RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution

    Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
    Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 79 kDa



     7.5% SDS-PAGE gel.

  • Anti-FACL4 antibody (ab227256) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Predicted band size: 79 kDa



    7.5% SDS-PAGE gel.

References

ab227256 has not yet been referenced specifically in any publications.

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