Anti-FACL4 antibody (ab227256)
Key features and details
- Rabbit polyclonal to FACL4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-FACL4 antibody
See all FACL4 primary antibodies -
Description
Rabbit polyclonal to FACL4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig, Rhesus monkey
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Immunogen
Recombinant fragment within Human FACL4 (internal sequence). The exact sequence is proprietary.
Database link: O60488 -
Positive control
- WB: HeLa, RAW 264.7, PC-12, MCF7, MDA-MB-231 and Rat2 whole cell extracts. IHC-P: Mouse kidney tissue; Rat duodenum tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 79 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. -
Involvement in disease
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2182 Human
- Entrez Gene: 50790 Mouse
- Entrez Gene: 448980 Pig
- Entrez Gene: 113976 Rat
- Omim: 300157 Human
- SwissProt: O60488 Human
- SwissProt: Q9QUJ7 Mouse
- SwissProt: O35547 Rat
see all -
Alternative names
- ACS 4 antibody
- ACS4 antibody
- ACSL 4 antibody
see all
Images
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Western blot - Anti-FACL4 antibody (ab227256)All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : FACL4 shRNA-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (ab227256)Paraffin-embedded mouse kidney tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody (ab227256)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FACL4 (green) using ab227256 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (ab227256)Paraffin-embedded rat duodenum tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-FACL4 antibody (ab227256)Anti-FACL4 antibody (ab227256) at 1/1000 dilution + RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-FACL4 antibody (ab227256)All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-FACL4 antibody (ab227256)All lanes : Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell extract
Lane 2 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-FACL4 antibody (ab227256)Anti-FACL4 antibody (ab227256) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 79 kDa7.5% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227256 has not yet been referenced specifically in any publications.
