Anti-FACL4 antibody - Carboxyterminal end (ab137525)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-FACL4 antibody - Carboxyterminal end
See all FACL4 primary antibodies -
Description
Rabbit polyclonal to FACL4 - Carboxyterminal end -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Pig
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Immunogen
Synthetic peptide, corresponding to a region within the C terminal amino acids 649-711 of Human FACL4 (NP_075266.1).
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Positive control
- Hela, NT2D1, NIH 3T3, JC, BCL1 whole cell lysates, HeLa cells, HBL435 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 79 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates. -
Involvement in disease
Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis. -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 2182 Human
- Entrez Gene: 50790 Mouse
- Entrez Gene: 448980 Pig
- Entrez Gene: 113976 Rat
- Omim: 300157 Human
- SwissProt: O60488 Human
- SwissProt: Q9QUJ7 Mouse
- SwissProt: O35547 Rat
see all -
Alternative names
- ACS 4 antibody
- ACS4 antibody
- ACSL 4 antibody
see all
Images
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Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)All lanes : Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : NT2D1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa
Additional bands at: 74 kDa (possible isoform)
7.5% SDS PAGE -
Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)All lanes : Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/5000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (ab137525)Immunohistochemical analysis of paraffin embedded HBL435 xenograft tissue labelling FACL4 with ab137525 at 1/500. -
Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (ab137525)Immunofluorescence analysis of methanol fixed HeLa cells labelling FACL4 with ab137525 at 1/500 (Green). Alpha tubulin filaments were labelled using a suitable antibody(Red).
References
This product has been referenced in:
- Ansari IH et al. Characterization of Acyl-CoA synthetase isoforms in pancreatic beta cells: Gene silencing shows participation of ACSL3 and ACSL4 in insulin secretion. Arch Biochem Biophys 618:32-43 (2017). Read more (PubMed: 28193492) »
- Gelmetti V et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy 13:654-669 (2017). Read more (PubMed: 28368777) »
