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Signal Transduction Signaling Pathway Lipid Signaling Other
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Anti-FACL4 antibody - Carboxyterminal end (ab137525)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (5)

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Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
  • Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
  • Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (ab137525)

Key features and details

  • Rabbit polyclonal to FACL4 - Carboxyterminal end
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Protein
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Recombinant Human FACL4 protein (ab152375)
Primary
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Secondary
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Overview

  • Product name

    Anti-FACL4 antibody - Carboxyterminal end
    See all FACL4 primary antibodies
  • Description

    Rabbit polyclonal to FACL4 - Carboxyterminal end
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 649-711 of Human FACL4 (NP_075266.1).

  • Positive control

    • Hela, NT2D1, NIH 3T3, JC, BCL1 whole cell lysates, HeLa cells, HBL435 xenograft tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Lipid Signaling
    • Other
    • Signal Transduction
    • Metabolism
    • Lipid metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • HeLa membrane extract lysate (ab29547)
    • NIH/3T3 whole cell lysate (ab7179)
  • Recombinant Protein

    • Recombinant Human FACL4 protein (ab152375)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/1000.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
ICC/IF (1)
1/100 - 1/1000.
Notes
IHC-P
1/100 - 1/1000.
WB
1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
  • Involvement in disease

    Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession O60488 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2182 Human
    • Entrez Gene: 50790 Mouse
    • Entrez Gene: 448980 Pig
    • Entrez Gene: 113976 Rat
    • Omim: 300157 Human
    • SwissProt: O60488 Human
    • SwissProt: Q9QUJ7 Mouse
    • SwissProt: O35547 Rat
    • Unigene: 268785 Human
    • Unigene: 391337 Mouse
    • Unigene: 87821 Rat
    see all
  • Alternative names

    • ACS 4 antibody
    • ACS4 antibody
    • ACSL 4 antibody
    • Acsl4 antibody
    • ACSL4_HUMAN antibody
    • acyl CoA synthetase 4 antibody
    • Acyl CoA synthetase long chain family member 4 antibody
    • FACL 4 antibody
    • FACL4 antibody
    • Fatty acid Coenzyme A ligase antibody
    • fatty acid Coenzyme A ligase long-chain 4 antibody
    • LACS 4 antibody
    • LACS4 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain 4 antibody
    • long chain acyl CoA synthetase 4 antibody
    • long chain fatty acid CoA ligase 4 antibody
    • long chain fatty acid Coenzyme A ligase 4 antibody
    • Long-chain acyl-CoA synthetase 4 antibody
    • Long-chain-fatty-acid--CoA ligase 4 antibody
    • MRX63 antibody
    • MRX68 antibody
    see all

Images

  • Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    All lanes : Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : NT2D1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa
    Additional bands at: 74 kDa (possible isoform)



    7.5% SDS PAGE
  • Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    All lanes : Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/5000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    Immunohistochemical analysis of paraffin embedded HBL435 xenograft tissue labelling FACL4 with ab137525 at 1/500.
  • Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (ab137525)
    Immunofluorescence analysis of methanol fixed HeLa cells labelling FACL4 with ab137525 at 1/500 (Green). Alpha tubulin filaments were labelled using a suitable antibody(Red).

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab137525? Please let us know so that we can cite the reference in this datasheet.

ab137525 has been referenced in 5 publications.

  • Xiao X  et al. miR-212-5p attenuates ferroptotic neuronal death after traumatic brain injury by targeting Ptgs2. Mol Brain 12:78 (2019). PubMed: 31533781
  • Ansari IH  et al. Characterization of Acyl-CoA synthetase isoforms in pancreatic beta cells: Gene silencing shows participation of ACSL3 and ACSL4 in insulin secretion. Arch Biochem Biophys 618:32-43 (2017). PubMed: 28193492
  • Gelmetti V  et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy 13:654-669 (2017). PubMed: 28368777
  • Van Laar VS  et al. Glutamate excitotoxicity in neurons triggers mitochondrial and endoplasmic reticulum accumulation of Parkin, and, in the presence of N-acetyl cysteine, mitophagy. Neurobiol Dis 74:180-93 (2015). PubMed: 25478815
  • Wu X  et al. Long chain fatty Acyl-CoA synthetase 4 is a biomarker for and mediator of hormone resistance in human breast cancer. PLoS One 8:e77060 (2013). WB ; Human . PubMed: 24155918

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-FACL4 antibody - Carboxyterminal end

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y CELLS)
Permeabilization
Yes - 0.3% TRITON X
Specification
SH-SY5Y CELLS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Read More

Dr. Anu Antony

Verified customer

Submitted Feb 26 2018

Western blot abreview for Anti-FACL4 antibody - Carboxyterminal end

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - other (Proteins extracted from MAM complex isolated from)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
25 µg
Specification
Proteins extracted from MAM complex isolated from
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Dr. Anu Antony

Verified customer

Submitted Feb 26 2018

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