Overview

  • Product name
    Anti-FACL4 antibody - Carboxyterminal end
    See all FACL4 primary antibodies
  • Description
    Rabbit polyclonal to FACL4 - Carboxyterminal end
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 649-711 of Human FACL4 (NP_075266.1).

  • Positive control
    • Hela, NT2D1, NIH 3T3, JC, BCL1 whole cell lysates, HeLa cells, HBL435 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 79 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
  • Involvement in disease
    Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
  • Sequence similarities
    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization
    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACS 4 antibody
    • ACS4 antibody
    • ACSL 4 antibody
    • Acsl4 antibody
    • ACSL4_HUMAN antibody
    • acyl CoA synthetase 4 antibody
    • Acyl CoA synthetase long chain family member 4 antibody
    • FACL 4 antibody
    • FACL4 antibody
    • Fatty acid Coenzyme A ligase antibody
    • fatty acid Coenzyme A ligase long-chain 4 antibody
    • LACS 4 antibody
    • LACS4 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain 4 antibody
    • long chain acyl CoA synthetase 4 antibody
    • long chain fatty acid CoA ligase 4 antibody
    • long chain fatty acid Coenzyme A ligase 4 antibody
    • Long-chain acyl-CoA synthetase 4 antibody
    • Long-chain-fatty-acid--CoA ligase 4 antibody
    • MRX63 antibody
    • MRX68 antibody
    see all

Images

  • All lanes : Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : NT2D1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa
    Additional bands at: 74 kDa (possible isoform)



    7.5% SDS PAGE
  • All lanes : Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/5000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded HBL435 xenograft tissue labelling FACL4 with ab137525 at 1/500.
  • Immunofluorescence analysis of methanol fixed HeLa cells labelling FACL4 with ab137525 at 1/500 (Green). Alpha tubulin filaments were labelled using a suitable antibody(Red).

References

This product has been referenced in:
  • Ansari IH  et al. Characterization of Acyl-CoA synthetase isoforms in pancreatic beta cells: Gene silencing shows participation of ACSL3 and ACSL4 in insulin secretion. Arch Biochem Biophys 618:32-43 (2017). Read more (PubMed: 28193492) »
  • Gelmetti V  et al. PINK1 and BECN1 relocalize at mitochondria-associated membranes during mitophagy and promote ER-mitochondria tethering and autophagosome formation. Autophagy 13:654-669 (2017). Read more (PubMed: 28368777) »
See all 4 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y CELLS)
Permeabilization
Yes - 0.3% TRITON X
Specification
SH-SY5Y CELLS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Anu Shanu

Verified customer

Submitted Feb 26 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (Proteins extracted from MAM complex isolated from)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
25 µg
Specification
Proteins extracted from MAM complex isolated from
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Anu Shanu

Verified customer

Submitted Feb 26 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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