Recombinant
RabMAb

Recombinant Anti-FACL4 antibody [EPR8640] - BSA and Azide free (ab240135)

Overview

  • Product name

    Anti-FACL4 antibody [EPR8640] - BSA and Azide free
    See all FACL4 primary antibodies
  • Description

    Rabbit monoclonal [EPR8640] to FACL4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human FACL4. The exact sequence is proprietary.

  • General notes

    Ab240135 is the carrier-free version of ab155282. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab240135 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 79 kDa.

Target

  • Function

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses arachidonate and eicosapentaenoate as substrates.
  • Involvement in disease

    Defects in ACSL4 are the cause of mental retardation X-linked type 63 (MRX63) [MIM:300387]. Mental retardation is a mental disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Non-syndromic mental retardation patients do not manifest other clinical signs.
    Defects in ACSL4 are involved in Alport syndrome with mental retardation midface hypoplasia and elliptocytosis (ATS-MR) [MIM:300194]. A X-linked contiguous gene deletion syndrome characterized by glomerulonephritis, deafness, mental retardation, midface hypoplasia and elliptocytosis.
  • Sequence similarities

    Belongs to the ATP-dependent AMP-binding enzyme family.
  • Cellular localization

    Mitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACS 4 antibody
    • ACS4 antibody
    • ACSL 4 antibody
    • Acsl4 antibody
    • ACSL4_HUMAN antibody
    • acyl CoA synthetase 4 antibody
    • Acyl CoA synthetase long chain family member 4 antibody
    • FACL 4 antibody
    • FACL4 antibody
    • Fatty acid Coenzyme A ligase antibody
    • fatty acid Coenzyme A ligase long-chain 4 antibody
    • LACS 4 antibody
    • LACS4 antibody
    • Lignoceroyl CoA synthase antibody
    • Long chain 4 antibody
    • long chain acyl CoA synthetase 4 antibody
    • long chain fatty acid CoA ligase 4 antibody
    • long chain fatty acid Coenzyme A ligase 4 antibody
    • Long-chain acyl-CoA synthetase 4 antibody
    • Long-chain-fatty-acid--CoA ligase 4 antibody
    • MRX63 antibody
    • MRX68 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FACL4 with purified ab155282 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155282).

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FACL4 with purified ab155282 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/200) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155282).

  • Flow Cytometry analysis of 293T cells labelling FACL4 with purified ab155282 at 1/100 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155282).

  • ab155282 (purified) at 1/30 immunoprecipitating FACL4 in HepG2 whole cell lysate.

    Lane 1 (input): HepG2 whole cell lysate (10µg)

    Lane 2 (+): ab155282 + HepG2 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155282 in HepG2 whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155282).

  • Flow cytometric analysis of permeabilized 293T cells labelling FACL4 with unpurified ab155282 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155282).

References

ab240135 has not yet been referenced specifically in any publications.

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