The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/1000. Predicted molecular weight: 85 kDa.
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
Involvement in disease
Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Contains 3 Sushi (CCP/SCR) domains. Contains 1 VWFA domain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (ab176024)
Immunohistochemical analysis of formalin fixed, paraffin embedded Human hepatocarcinoma tissue labeling Factor B with ab176024 at 1/50, followed by peroxidase conjugation of the secondary antibody and DAB staining
Western blot - Anti-Factor B antibody (ab176024)
All lanes : Anti-Factor B antibody (ab176024) at 1/1000 dilution
Lane 1 : HepG2 cell line lysate Lane 2 : Mouse liver tissue lysate