Overview

  • Product name

  • Description

    Rabbit polyclonal to Factor B
  • Host species

    Rabbit
  • Specificity

    ab72658 detects a fragment from activated Factor B; the Bb fragment, resulting from cleavage adjacent to Lys260.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide:

    KIVLDPSGSMN IYLVLDGSD

    , corresponding to amino acids 260-280 of Human Factor B.

  • Positive control

    • Extracts from K562 cells, treated with etoposide (25uM, 1hour).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).
ELISA 1/20000.

Target

  • Function

    Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.
  • Involvement in disease

    Defects in CFB are a cause of susceptibility to hemolytic uremic syndrome atypical type 4 (AHUS4) [MIM:612924]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
    Contains 3 Sushi (CCP/SCR) domains.
    Contains 1 VWFA domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHUS4 antibody
    • B factor properdin antibody
    • BF antibody
    • BFD antibody
    • C3 proaccelerator antibody
    • C3 proactivator antibody
    • C3/C5 convertase antibody
    • CFAB antibody
    • CFAB_HUMAN antibody
    • CFB antibody
    • Complement Factor B antibody
    • Complement factor B Bb fragment antibody
    • FB antibody
    • FBI12 antibody
    • GBG antibody
    • Glycine rich beta glycoprotein antibody
    • Glycine-rich beta glycoprotein antibody
    • H2 Bf antibody
    • H2BF antibody
    • PBF2 antibody
    • Properdin factor B antibody
    see all

Images

  • All lanes : Anti-Factor B antibody (ab72658) at 1/200 dilution

    Lane 1 : Extracts from K562 cells, treated with etoposide (25uM, 1hour)
    Lane 2 : Extracts from K562 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 54 and 86 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 40 kDa (possible non-specific binding), 75 kDa. We are unsure as to the identity of these extra bands.

References

ab72658 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

DISCOUNT CODE: ********
Expiration date: *******

I am very pleased to hear you would like to accept our offer and test ab72658 in mouse tissue. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for mouse tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your interest in ab72658. I have confirmed with the laboratory that this antibody is specific for the CFAB Bb which is cleaved at Lys260. It cannot cross react with Factor-Ba. I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up