Overview

  • Product name

    Anti-Factor D/CFD antibody [EPR14699]
    See all Factor D/CFD primary antibodies
  • Description

    Rabbit monoclonal [EPR14699] to Factor D/CFD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Factor D/CFD aa 50-250. The exact sequence is proprietary.
    Database link: P00746

  • Positive control

    • WB: Human serum; THP-1, U87-MG and Molt4 cell lysates
  • General notes

     

     

     This product was previously labelled as Factor D

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14699
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab204917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).

Target

  • Function

    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • Involvement in disease

    Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adipsin antibody
    • Adipsin/complement factor D antibody
    • Adn antibody
    • C3 convertase activator antibody
    • CFAD_HUMAN antibody
    • CFD antibody
    • Complement factor D antibody
    • complement factor D preproprotein antibody
    • D component of complement antibody
    • DF antibody
    • FactorD antibody
    • PFD antibody
    • Properdin factor D antibody
    see all

Images

  • All lanes : Anti-Factor D/CFD antibody [EPR14699] (ab204917) at 1/2000 dilution

    Lane 1 : Human serum
    Lane 2 : THP-1 (Human monocytic leukemia cells) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

  • All lanes : Anti-Factor D/CFD antibody [EPR14699] (ab204917) at 1/1000 dilution

    Lane 1 : Human serum
    Lane 2 : THP-1 (Human monocytic leukemia cells) cell lysate
    Lane 3 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
    Lane 4 : Molt4 (Human lymphoblastic leukemia cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 27 kDa
    Observed band size: 25 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    5% NFDM/TBST: Blocking and diluting buffer.

References

ab204917 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab204917.
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