Recombinant
RabMAb

Recombinant Anti-Factor D/CFD antibody [EPR17004-240] (ab213177)

Overview

  • Product name

    Anti-Factor D/CFD antibody [EPR17004-240]
    See all Factor D/CFD primary antibodies
  • Description

    Rabbit monoclonal [EPR17004-240] to Factor D/CFD
  • Host species

    Rabbit
  • Specificity

    This antibody detects a distinct epitope compared to ab181307.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein within Mouse Factor D/CFD aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P03953

  • Positive control

    • WB: His-tagged mouse Factor D/CFD (aa26-259) recombinant protein; Mouse plasma and serum; Mouse lung lysate.
  • General notes

     This product was previously labelled as Factor D

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17004-240
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab213177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 45-50 kDa (predicted molecular weight: 28 kDa).

Target

  • Function

    Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway.
  • Involvement in disease

    Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 1 peptidase S1 domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Adipsin antibody
    • Adipsin/complement factor D antibody
    • Adn antibody
    • C3 convertase activator antibody
    • CFAD_HUMAN antibody
    • CFD antibody
    • Complement factor D antibody
    • complement factor D preproprotein antibody
    • D component of complement antibody
    • DF antibody
    • FactorD antibody
    • PFD antibody
    • Properdin factor D antibody
    see all

Images

  • Anti-Factor D/CFD antibody [EPR17004-240] (ab213177) at 1/5000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa
    Observed band size: 29 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Factor D/CFD antibody [EPR17004-240] (ab213177) at 1/1000 dilution

    Lane 1 : Mouse plasma
    Lane 2 : Mouse serum
    Lane 3 : Mouse lung lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa
    Observed band size: 45-50 kDa why is the actual band size different from the predicted?



     

    Exposure : Lane 1/2: 1 second; Lane 3: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The higher molecular mass corresponds to the glycosylated protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; PMID: 12949072).

References

ab213177 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213177.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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