Recombinant Anti-Factor D/CFD antibody [EPR17004-285] (ab181307)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17004-285] to Factor D/CFD
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Factor D/CFD antibody [EPR17004-285]
See all Factor D/CFD primary antibodies -
Description
Rabbit monoclonal [EPR17004-285] to Factor D/CFD -
Host species
Rabbit -
Specificity
This antibody detects a distinct epitope compared to ab213177. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein. Mouse plasma, serum and lung lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17004-285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 27 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 27 kDa. |
Target
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Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
Involvement in disease
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11537 Mouse
- SwissProt: P03953 Mouse
- Unigene: 4407 Mouse
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Alternative names
- Adipsin antibody
- Adipsin/complement factor D antibody
- Adn antibody
see all
Images
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Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/100000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/ Dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse serum lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.
Exposure times:
Lane 1/2: 1 second
Lane 3: 15 seconds
The higher molecular mass corresponds to glycosylated forms of the protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; PMID: 12949072).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181307 has not yet been referenced specifically in any publications.