Recombinant Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free (ab250478)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17004-285] to Factor D/CFD - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Factor D/CFD antibody [EPR17004-285] - BSA and Azide free
See all Factor D/CFD primary antibodies -
Description
Rabbit monoclonal [EPR17004-285] to Factor D/CFD - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody detects a distinct epitope compared to ab213177.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250478 is the carrier-free version of ab181307.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17004-285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 27 kDa. |
Target
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Function
Factor D cleaves factor B when the latter is complexed with factor C3b, activating the C3bbb complex, which then becomes the C3 convertase of the alternate pathway. Its function is homologous to that of C1s in the classical pathway. -
Involvement in disease
Defects in CFD are the cause of complement factor D deficiency (CFD deficiency) [MIM:134350]. CFD deficiency predisposes to invasive meningococcal disease. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11537 Mouse
- SwissProt: P03953 Mouse
- Unigene: 4407 Mouse
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Alternative names
- Adipsin antibody
- Adipsin/complement factor D antibody
- Adn antibody
see all
Images
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Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/100000 dilution + His-tagged Mouse Factor D/CFD (aa26-259) recombinant protein 10 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 29 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThis data was developed using ab181307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Factor D/CFD antibody [EPR17004-285] (ab181307) at 1/1000 dilution
Lane 1 : Mouse plasma lysate
Lane 2 : Mouse serum lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 45-50 kDa why is the actual band size different from the predicted?This data was developed using ab181307, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 1 second; Lane 3: 15 seconds.
The higher molecular mass corresponds to glycosylated forms of the protein. The expression profile observed is consistent with what has been described in the literature (PMID: 20038603; 12949072).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250478 has not yet been referenced specifically in any publications.