Key features and details
- Rabbit polyclonal to Factor I/CFI
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Factor I/CFI antibody
See all Factor I/CFI primary antibodies
DescriptionRabbit polyclonal to Factor I/CFI
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Mouse Factor I/CFI aa 358-603. (Expressed in E.coli).
RKRVIGGKPANVGDYPWQVAIKDGQRITCGGIYIGGCWILTAAHCVRPSR AHSYQVWTALLDWLKPNSQLGIQTVKRVIVHEKYNGATFQNDIALIEMKM HTGKKECELPNSVPACVPWSPYLFQPNDRCIISGWGRGKDNQKVYSLRWG EVDLIGNCSQFYPDRYYEKEMQCAGTRDGSIDACKGDSGGPLVCEDINNV TYVWGIVSWGENCGKPEFPGVYTRVANYFDWISYHVGRSLVSQHNV
Database link: Q61129
- IHC-P: Mouse kidney tissue. WB: Recombinant mouse Factor I/CFI protein; Rat and mouse liver lysates; Mouse small intestine lysate.
This product was previously labelled as Factor I
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231969 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 67 kDa.|
FunctionResponsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.
Involvement in diseaseDefects in CFI are a cause of susceptibility to hemolytic uremic syndrome atypical type 3 (AHUS3) [MIM:612923]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Defects in CFI are the cause of complement factor I deficiency (CFI deficiency) [MIM:610984]. CFI deficiency is an autosomal recessive condition associated with a propensity to pyogenic infections.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 Kazal-like domain.
Contains 2 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
Contains 1 SRCR domain.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- AHUS3 antibody
- ARMD13 antibody
- C3b INA antibody
All lanes : Anti-Factor I/CFI antibody (ab231969) at 1 µg/ml
Lane 1 : Mouse liver lysate
Lane 2 : Mouse intestine lysate
Lane 3 : Rat liver lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 67 kDa
Anti-Factor I/CFI antibody (ab231969) at 2 µg/ml + Recombinant fragment of mouse Factor I/CFI protein
Predicted band size: 67 kDa
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Factor I/CFI using ab231969 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231969 has not yet been referenced specifically in any publications.