Key features and details
- Mouse monoclonal [1C2] to Factor IX/PTC
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Factor IX/PTC antibody [1C2]
See all Factor IX/PTC primary antibodies
DescriptionMouse monoclonal [1C2] to Factor IX/PTC
This antibody has specificity for native human Factor IX/PTC.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Factor IX/PTC.
EpitopeEpitope specificity of this antibody differs to that of ab17197 as determined by inhibition ELISA.
This product was previously labelled as Factor IX
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Constituents: 0.0268% PBS, 2.9% Sodium chloride
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab17196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 51 kDa.|
|IHC-P||1/50 - 1/200.|
FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Tissue specificitySynthesized primarily in the liver and secreted in plasma.
Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
modificationsActivated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Christmas Disease antibody
- Christmas factor antibody
- Coagulant factor IX antibody
All lanes : Anti-Factor IX/PTC antibody [1C2] (ab17196) at 5 µg/ml
Lane 1 : Human small intestine tissue lysate - total protein (ab29276)
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg/ml per lane.
Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
ab17196 has not yet been referenced specifically in any publications.