Key features and details
- Sheep polyclonal to Factor IX/PTC
- Suitable for: ELISA, IHC-P, Neutralising
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Factor IX/PTC antibody
See all Factor IX/PTC primary antibodies
DescriptionSheep polyclonal to Factor IX/PTC
Tested applicationsSuitable for: ELISA, IHC-P, Neutralisingmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Factor IX/PTC.
- Human Liver tissue
This product was previously labelled as Factor IX
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Constituents: 0.24% HEPES, 0.88% Sodium chloride, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab128048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|IHC-P||Use a concentration of 2.5 µg/ml.|
|Neutralising||Use at an assay dependent dilution. 60 Bethesda Units/ml IgG against normal plasma. One Bethesda unit/ml is defined as the amount of inhibitor that resulted in 50% residual F. IX activity after 2 hours at 37°C (Kasper CK et al, Thromb Diath Haemorrh 34:869, 1975).|
FunctionFactor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Tissue specificitySynthesized primarily in the liver and secreted in plasma.
Involvement in diseaseDefects in F9 are the cause of recessive X-linked hemophilia B (HEMB) [MIM:306900]; also known as Christmas disease.
Note=Mutations in position 43 (Oxford-3, San Dimas) and 46 (Cambridge) prevents cleavage of the propeptide, mutation in position 93 (Alabama) probably fails to bind to cell membranes, mutation in position 191 (Chapel-Hill) or in position 226 (Nagoya OR Hilo) prevent cleavage of the activation peptide.
Defects in F9 are the cause of thrombophilia due to factor IX defect (THR-FIX) [MIM:300807]. A hemostatic disorder characterized by a tendency to thrombosis.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
DomainCalcium binds to the gamma-carboxyglutamic acid (Gla) residues and, with stronger affinity, to another site, beyond the Gla domain.
modificationsActivated by factor XIa, which excises the activation peptide.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Christmas Disease antibody
- Christmas factor antibody
- Coagulant factor IX antibody
ab128048 has not yet been referenced specifically in any publications.