Overview

  • Product name

  • Description

    Rabbit polyclonal to Factor V
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Double Immunodiffusion, Immunodiffusion, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Factor V. (Isolated from pooled human plasma).
    Database link: P12259

  • Positive control

    • Human plasma.
  • General notes

    Measured by quantitative precipitin analysis the amount of Factor V precipitated by 1 ml antiserum is between 8 and 12 U. One Unit of Factor V is defined as the amount of Factor V present in 1 ml normal plasma. On average this corresponds to 20 μg.

Properties

  • Form

    Lyophilised:Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide

    No foreign proteins added.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Purification notes

    Delipidated, heat inactivated, stable whole antiserum, dialyzed against glycine buffer. Total protein and IgG concentrations in ab182958 are comparable to those of pooled normal rabbit serum.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Double Immunodiffusion Use at an assay dependent concentration.

In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Immunodiffusion Use at an assay dependent concentration.

In electroimmunodiffusion the antiserum concentration required in the gel is normally between 1 and 2%.

IP Use at an assay dependent concentration.

Target

  • Function

    Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Factor V deficiency
    Thrombophilia due to activated protein C resistance
    Budd-Chiari syndrome
    Ischemic stroke
    Pregnancy loss, recurrent, 1
  • Sequence similarities

    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • Domain

    Domain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats.
  • Post-translational
    modifications

    Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
    Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
    Activated protein C inactivates factor V and factor Va by proteolytic degradation.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activated protein C cofactor antibody
    • APC cofactor antibody
    • coagulation factor V (proaccelerin, labile factor) antibody
    • Coagulation factor V antibody
    • coagulation factor V jinjiang A2 domain antibody
    • Coagulation factor V light chain antibody
    • F5 antibody
    • FA5_HUMAN antibody
    • Factor V Leiden antibody
    • FactorV antibody
    • FVL antibody
    • Labile factor antibody
    • PCCF antibody
    • Proaccelerin antibody
    • proaccelerin, labile factor antibody
    • Protein C cofactor antibody
    • RPRGL1 antibody
    • THPH2 antibody
    see all

References

ab182958 has not yet been referenced specifically in any publications.

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