Product nameAnti-Factor V antibody
See all Factor V primary antibodies
DescriptionRabbit polyclonal to Factor V
Tested applicationsSuitable for: Double Immunodiffusion, Immunodiffusion, IPmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Factor V. (Isolated from pooled human plasma).
Database link: P12259
- Human plasma.
Measured by quantitative precipitin analysis the amount of Factor V precipitated by 1 ml antiserum is between 8 and 12 U. One Unit of Factor V is defined as the amount of Factor V present in 1 ml normal plasma. On average this corresponds to 20 μg.
FormLyophilised:Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
No foreign proteins added.
Concentration information loading...
Purification notesDelipidated, heat inactivated, stable whole antiserum, dialyzed against glycine buffer. Total protein and IgG concentrations in ab182958 are comparable to those of pooled normal rabbit serum.
Our Abpromise guarantee covers the use of ab182958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Double Immunodiffusion||Use at an assay dependent concentration.
In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter center well and 2 μl plasma samples (neat and serially diluted) in 2 mm diameter peripheral wells.
|Immunodiffusion||Use at an assay dependent concentration.
In electroimmunodiffusion the antiserum concentration required in the gel is normally between 1 and 2%.
|IP||Use at an assay dependent concentration.|
FunctionCentral regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Involvement in diseaseFactor V deficiency
Thrombophilia due to activated protein C resistance
Pregnancy loss, recurrent, 1
Sequence similaritiesBelongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains.
DomainDomain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats.
modificationsThrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
Activated protein C inactivates factor V and factor Va by proteolytic degradation.
Phosphorylation sites are present in the extracellular medium.
- Information by UniProt
- Activated protein C cofactor antibody
- APC cofactor antibody
- coagulation factor V (proaccelerin, labile factor) antibody
ab182958 has not yet been referenced specifically in any publications.