- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Factor VII antibody
See all Factor VII primary antibodies -
DescriptionSheep polyclonal to Factor VII
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Host speciesSheep
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Specificityab104564 is specific for Factor VII as determined by immunodiffusion. Identity was established with purified Factor VII.
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Tested applicationsSuitable for: IP, ELISA, IHC-P, Neutralisingmore details
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Species reactivityReacts with: Human
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Immunogen
Human Factor VII purified from plasma.
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Positive control
- Human Liver tissue
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 50% Glycerol, HEPES, pH 7.4 -
Concentration information loading... -
PurityAmmonium Sulphate Precipitation
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab104564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent dilution. | |
| ELISA | Use at an assay dependent dilution. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| Neutralising | Use at an assay dependent dilution. |
Target
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FunctionInitiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
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Tissue specificityPlasma.
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Involvement in diseaseDefects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.
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Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 2155 Human
- Omim: 613878 Human
- SwissProt: P08709 Human
- Unigene: 36989 Human
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Alternative names
- coagulation factor VII (serum prothrombin conversion accelerator) antibody
- Coagulation factor VII antibody
- Eptacog alfa antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VII antibody (ab104564)ab104564, at 5 ug/ml, staining Factor VII in formalin-fixed, paraffin-embedded Human Liver by Immunohistochemistry, using a biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References
ab104564 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
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