Anti-Factor VII antibody - C-terminal (ab197656)
Key features and details
- Rabbit polyclonal to Factor VII - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Factor VII antibody - C-terminal
See all Factor VII primary antibodies -
Description
Rabbit polyclonal to Factor VII - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Fusion protein within Human Factor VII (C terminal). The exact sequence is proprietary. Fusion protein corresponding to residues near the C terminal of human Factor VII Isoform B. NCBI Accession No. BC130468. The protein fusion partner is GST.
Database link: P08709-2 -
Positive control
- 293T, Jurkat and mouse NIH 3T3. Human lung cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/400 - 1/2000. Predicted molecular weight: 49 kDa.
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Notes |
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IHC-P
1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/400 - 1/2000. Predicted molecular weight: 49 kDa. |
Target
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Function
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2155 Human
- Entrez Gene: 14068 Mouse
- Entrez Gene: 260320 Rat
- Omim: 613878 Human
- SwissProt: P08709 Human
- SwissProt: P70375 Mouse
- SwissProt: Q8K3U6 Rat
- Unigene: 36989 Human
see all -
Alternative names
- coagulation factor VII (serum prothrombin conversion accelerator) antibody
- Coagulation factor VII antibody
- Eptacog alfa antibody
see all
Images
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All lanes : Anti-Factor VII antibody - C-terminal (ab197656) at 1/400 dilution
Lane 1 : mouse NIH 3T3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 49 kDa
Exposure time: 30 seconds -
Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Factor VII using ab197656 at a 1/40 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab197656 has not yet been referenced specifically in any publications.