Overview

  • Product name

    Anti-Factor VII antibody - C-terminal
    See all Factor VII primary antibodies
  • Description

    Rabbit Polyclonal to Factor VII - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab197656 detects endogenous level of total Factor VII protein.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Fusion protein within Human Factor VII (C terminal). The exact sequence is proprietary. Fusion protein corresponding to residues near the C terminal of human Factor VII Isoform B. NCBI Accession No. BC130468. The protein fusion partner is GST.
    Database link: P08709-2

  • Positive control

    • 293T, Jurkat and mouse NIH 3T3. Human lung cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197656 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/400 - 1/2000. Predicted molecular weight: 49 kDa.

Target

  • Function

    Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • coagulation factor VII (serum prothrombin conversion accelerator) antibody
    • Coagulation factor VII antibody
    • Eptacog alfa antibody
    • F7 antibody
    • FA7_HUMAN antibody
    • Factor VII antibody
    • Factor VII heavy chain antibody
    • Factor VII light chain antibody
    • FVII coagulation protein antibody
    • OTTHUMP00000018733 antibody
    • OTTHUMP00000018734 antibody
    • Proconvertin antibody
    • Serum prothrombin conversion accelerator antibody
    • SPCA antibody
    see all

Images

  • All lanes : Anti-Factor VII antibody - C-terminal (ab197656) at 1/400 dilution

    Lane 1 : mouse NIH 3T3 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 49 kDa


    Exposure time: 30 seconds
  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling Factor VII using ab197656 at a 1/40 dilution.

References

ab197656 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Rat Serum (Wistar rat)
Gel Running Conditions
Non-reduced Denaturing (10 % gel)
Loading amount
20 µg
Specification
Wistar rat
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Jiyeong Bae

Verified customer

Submitted Oct 19 2015

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