Western blot abreview for Anti-Factor VII antibody - C-terminal

Excellent
Abreviews
Application
Western blot
Sample
Rat Serum (Wistar rat)
Gel Running Conditions
Non-reduced Denaturing (10 % gel)
Loading amount
20 µg
Specification
Wistar rat
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Tris-Buffered Saline and 0.1% Tween 20
Dilution
1/2000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: Anti-rabbit IgG, HRP-linked Antibody
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 50 kDa

Jiyeong Bae

Verified customer

Submitted Oct 19 2015

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