Overview

  • Product name

    Anti-Factor VII antibody [EP6185(2)]
    See all Factor VII primary antibodies
  • Description

    Rabbit monoclonal [EP6185(2)] to Factor VII
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Factor VII aa 150-250. The exact sequence is proprietary.

  • Positive control

    • Human placenta, serum, fetal liver and HepG2 lysates.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP6185(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level.
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • Post-translational
      modifications

      The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • coagulation factor VII (serum prothrombin conversion accelerator) antibody
      • Coagulation factor VII antibody
      • Eptacog alfa antibody
      • F7 antibody
      • FA7_HUMAN antibody
      • Factor VII antibody
      • Factor VII heavy chain antibody
      • Factor VII light chain antibody
      • FVII coagulation protein antibody
      • OTTHUMP00000018733 antibody
      • OTTHUMP00000018734 antibody
      • Proconvertin antibody
      • Serum prothrombin conversion accelerator antibody
      • SPCA antibody
      see all

    Images

    • All lanes : Anti-Factor VII antibody [EP6185(2)] (ab151543) at 1/1000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : Human serum lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa

    References

    ab151543 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab151543.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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