• Product name

  • Description

    Rabbit polyclonal to Factor VIIa
  • Host species

  • Tested applications

    Suitable for: Prothrombin Assay, Indirect ELISA, Inhibition Assaymore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein (Human) (NovoSeven®).

  • General notes

    0.02% sodium azide can be added as a preservative if storing at 4°C


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab7053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Prothrombin Assay
Indirect ELISA
Inhibition Assay
  • Application notes
    I-ELISA: 1/15000 for antibodies at 0.1 mg/ml to generate absorbance 1.0 at 450 nm after 10 minutes of incubation with OPD tablets at room temperature.
    Inhib: Use at an assay dependant dilution.
    PA: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
    • Tissue specificity

    • Involvement in disease

      Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. A hemorrhagic disease with variable presentation. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or repeated hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Finally, numerous subjects are completely asymptomatic despite very low factor VII levels.
    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 Gla (gamma-carboxy-glutamate) domain.
      Contains 1 peptidase S1 domain.
    • Post-translational

      The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
      The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
      O- and N-glycosylated. N-glycosylation at Asn-205 occurs cotranslationally and is mediated by STT3A-containing complexes, while glycosylation at Asn-382 is post-translational and is mediated STT3B-containing complexes before folding. O-fucosylated by POFUT1 on a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • Coagulation factor VII antibody
      • Eptacog alfa antibody
      • F7 antibody
      • FA7_HUMAN antibody
      • Factor VII heavy chain antibody
      • FVII coagulation protein antibody
      • Proconvertin antibody
      • Serum prothrombin conversion accelerator antibody
      • SPCA antibody
      see all


    This product has been referenced in:

    • Kamocka M  et al. Rat prostate tumors express cancer procoagulant, an activator of coagulation factor X. Comp Med 58:282-6 (2008). ELISA . Read more (PubMed: 18589871) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A


    Thank you for your enquiry. For ab21037, the antibody was tested against purified Human Factor V and against human plasma in ELISA and Western blot. The characterization of ab20951, MAb to Factor V (Clone 701), was described in the following published reference. Please note that this antibody is referred to as Clone B38 in the paper. Gewirtz AM et al. Biology of human megakaryocyte factor V. Blood 67:1639-48 (1986). PubMed: 2423159 Ab1009 and ab1015 were tested using Factor V purified from human plasma and rabbit anti-human Factor V antiserum. For ab7053, unfortunately, we do not have more information about this antibody. Please contact us again if you have any additional questions.

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    Thank you for your enquiry. Unfortunately, we do not have more information about this antibody. We will update the on-line datasheet of this product as soon as we get more data. We apologize for any inconvenience caused.

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    I'm afraid that we are unable to answer your customer's question. We know that this antibody cross reacts with Factor VII, but do not have any further information about the relative degree of cross reactivity.

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    No, this is our only antibody against factor VIIa

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    The only other species tested has been rat.

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    This antibody has not beed tested on the Western Blot. However, it binds very strongly to native Factor VIIa and Factor VII. No, the cross-reactivity has not been determined. Technical Support- AgriSera

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