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    factor-viii-antibody-274-ab41188.pdf

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Cardiovascular Blood Coagulation Intrinsic
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Anti-Factor VIII antibody [27.4] (ab41188)

  • Datasheet
Submit a review Q&A (3)References (3)

Product price, shipping and contact information

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Abpromise

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Key features and details

  • Mouse monoclonal [27.4] to Factor VIII
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG2a

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Recombinant Human Factor VIII protein (ab158403)
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Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)

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Overview

  • Product name

    Anti-Factor VIII antibody [27.4]
    See all Factor VIII primary antibodies
  • Description

    Mouse monoclonal [27.4] to Factor VIII
  • Host species

    Mouse
  • Specificity

    ab41188 does not cross react with the von Willebrand factor.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Factor VIII (N terminal).

  • Epitope

    ab41188 recognises an epitope in the N-terminal region of the 83kD light chain of Factor VIII.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from TCS
  • Clonality

    Monoclonal
  • Clone number

    27.4
  • Isotype

    IgG2a
  • Research areas

    • Cardiovascular
    • Blood
    • Coagulation
    • Intrinsic

Associated products

  • Assay kits

    • Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Recombinant Protein

    • Recombinant Human Factor VIII protein (ab158403)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab41188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
Notes
Application notes
ELISA: 1/16,000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 267 kDa.

Dilute in PBS or medium which is identical to that used in the assay system.

Can inhibit Factor VIII activity in coagulation assays.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

  • Function

    Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • Involvement in disease

    Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • Sequence similarities

    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • Domain

    Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • Post-translational
    modifications

    Sulfation on Tyr-1699 is essential for binding vWF.
  • Cellular localization

    Secreted > extracellular space.
  • Target information above from: UniProt accession P00451 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2157 Human
    • Omim: 300841 Human
    • SwissProt: P00451 Human
    • Unigene: 654450 Human
    • Alternative names

      • AHF antibody
      • Antihemophilic factor antibody
      • Coagulation factor VIII antibody
      • coagulation factor VIII, procoagulant component antibody
      • coagulation factor VIIIc antibody
      • DXS1253E antibody
      • F8 antibody
      • F8b antibody
      • F8c antibody
      • FA8_HUMAN antibody
      • factor VIII F8B antibody
      • Factor VIIIa light chain antibody
      • FactorVIII antibody
      • FVIII antibody
      • Hema antibody
      • Hemophilia A antibody
      • Hemophilia, classic antibody
      • OTTHUMP00000061446 antibody
      • OTTHUMP00000196174 antibody
      • Procoagulant component antibody
      see all

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (3)

    Publishing research using ab41188? Please let us know so that we can cite the reference in this datasheet.

    ab41188 has been referenced in 3 publications.

    • Gage BK  et al. Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Cell Stem Cell 27:254-269.e9 (2020). IHC-P ; Human . PubMed: 32640183
    • Jankowska KI  et al. Further Evidence That MicroRNAs Can Play a Role in Hemophilia A Disease Manifestation: F8 Gene Downregulation by miR-19b-3p and miR-186-5p. Front Cell Dev Biol 8:669 (2020). PubMed: 32850803
    • Pandey GS  et al. Detection of intracellular Factor VIII protein in peripheral blood mononuclear cells by flow cytometry. Biomed Res Int 2013:793502 (2013). PubMed: 23555096

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Question

    Thanks a lot for your help.   When we should expect the delivery of ab41186 antibody to our lab?  

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    You will receive this replacement vial tomorrow.

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Question

            Dear Jeremy,   Since the ab41188 is not working properly in our system. We would like to replace this antibody with ab41186, which is IgG1 isotype.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    Sorry about that. I did set up the order for the correct replacement antibody, 1 vial of ab41186, but noted the wrong catalog number in the email to you.    

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Question

    As we talked on the phone regarding the problem I found with the anti-factor 8 (cat.no. ab41188). The antibody (mIgG2a) is falling of from protein A biosensors analysed by Octet (as attached and we found the same pattern in the other two experiments). From our discussion, you kindly suggested us to replace the antibody I am using now with a new tubes and it can be the same or different catalog no.  After a long discussion and a new trial with a new set of biosensors. We kept discover the same results, this antibody (the cat. No. ab41188) is falling off from protein A and G. Therefore here, we decided to replace the anti-factor 8 cat.no. ab41188 with anti-factor 8 cat.no. ab41186.   The one that we are using now was ordered on 08/11/2011, confirmation number 931995 Please let me know if you need any other information.     Thank you/ Best,   Wanida C Hollis Division of Hematology (HFM-330) Center for Biologics Evaluation and Research Food and Drug Administration NIH Building 29, Room 328 Tel (301) 8272498 · Fax (301) 402-2780          

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2011

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41188 with the order number 973153. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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