Anti-Factor VIII antibody [27.4] (ab41188)

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Key features and details

  • Mouse monoclonal [27.4] to Factor VIII
  • Suitable for: ELISA, WB
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Factor VIII antibody [27.4]
    See all Factor VIII primary antibodies

  • Description

    Mouse monoclonal [27.4] to Factor VIII

  • Host species

    Mouse

  • Specificity

    ab41188 does not cross react with the von Willebrand factor.

  • Tested applications

    Suitable for: ELISA, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    N-terminal region of the 83kD light chain of purified human Factor VIII.

  • Epitope

    ab41188 recognises an epitope in the N-terminal region of the 83kD light chain of Factor VIII.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • Storage buffer

    pH: 7.40
    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS

  • Clonality

    Monoclonal

  • Clone number

    27.4

  • Isotype

    IgG2a

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/16,000.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 267 kDa.

    Dilute in PBS or medium which is identical to that used in the assay system.

    Can inhibit Factor VIII activity in coagulation assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function

      Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.

    • Involvement in disease

      Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.

    • Sequence similarities

      Belongs to the multicopper oxidase family.
      Contains 3 F5/8 type A domains.
      Contains 2 F5/8 type C domains.
      Contains 6 plastocyanin-like domains.

    • Domain

      Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.

    • Post-translational
      modifications

      Sulfation on Tyr-1699 is essential for binding vWF.

    • Cellular localization

      Secreted > extracellular space.
    • Information by UniProt

    • Database links

      • Alternative names

        • AHF antibody
        • Antihemophilic factor antibody
        • Coagulation factor VIII antibody
        • coagulation factor VIII, procoagulant component antibody
        • coagulation factor VIIIc antibody
        • DXS1253E antibody
        • F8 antibody
        • F8b antibody
        • F8c antibody
        • FA8_HUMAN antibody
        • factor VIII F8B antibody
        • Factor VIIIa light chain antibody
        • FactorVIII antibody
        • FVIII antibody
        • Hema antibody
        • Hemophilia A antibody
        • Hemophilia, classic antibody
        • OTTHUMP00000061446 antibody
        • OTTHUMP00000196174 antibody
        • Procoagulant component antibody
        see all

      References (2)

      Publishing research using ab41188? Please let us know so that we can cite the reference in this datasheet.

      ab41188 has been referenced in 2 publications.

      • Gage BK  et al. Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts. Cell Stem Cell 27:254-269.e9 (2020). IHC-P ; Human . PubMed: 32640183
      • Pandey GS  et al. Detection of intracellular Factor VIII protein in peripheral blood mononuclear cells by flow cytometry. Biomed Res Int 2013:793502 (2013). PubMed: 23555096

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      Thanks a lot for your help.   When we should expect the delivery of ab41186 antibody to our lab?  

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      Answer

      You will receive this replacement vial tomorrow.

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      Question

              Dear Jeremy,   Since the ab41188 is not working properly in our system. We would like to replace this antibody with ab41186, which is IgG1 isotype.

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      Answer

      Sorry about that. I did set up the order for the correct replacement antibody, 1 vial of ab41186, but noted the wrong catalog number in the email to you.    

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      Question

      As we talked on the phone regarding the problem I found with the anti-factor 8 (cat.no. ab41188). The antibody (mIgG2a) is falling of from protein A biosensors analysed by Octet (as attached and we found the same pattern in the other two experiments). From our discussion, you kindly suggested us to replace the antibody I am using now with a new tubes and it can be the same or different catalog no.  After a long discussion and a new trial with a new set of biosensors. We kept discover the same results, this antibody (the cat. No. ab41188) is falling off from protein A and G. Therefore here, we decided to replace the anti-factor 8 cat.no. ab41188 with anti-factor 8 cat.no. ab41186.   The one that we are using now was ordered on 08/11/2011, confirmation number 931995 Please let me know if you need any other information.     Thank you/ Best,   Wanida C Hollis Division of Hematology (HFM-330) Center for Biologics Evaluation and Research Food and Drug Administration NIH Building 29, Room 328 Tel (301) 8272498 · Fax (301) 402-2780          

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      Answer

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab41188 with the order number 973153. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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