Overview

  • Product name
  • Description
    Sheep polyclonal to Factor VIII
  • Host species
    Sheep
  • Specificity
    No reactivity was observed with von Willebrand Factor.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, Immunodiffusionmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human Factor VIII purified from Factor VIII concentrate.

  • Positive control
    • Human skeletal muscle and capillaries

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.40
    Constituents: 0.24% HEPES, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab139391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Immunodiffusion Use at an assay dependent concentration.

Target

  • Function
    Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • Involvement in disease
    Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • Sequence similarities
    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • Domain
    Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • Post-translational
    modifications
    Sulfation on Tyr-1699 is essential for binding vWF.
  • Cellular localization
    Secreted > extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • AHF antibody
    • Antihemophilic factor antibody
    • Coagulation factor VIII antibody
    • coagulation factor VIII, procoagulant component antibody
    • coagulation factor VIIIc antibody
    • DXS1253E antibody
    • F8 antibody
    • F8b antibody
    • F8c antibody
    • FA8_HUMAN antibody
    • factor VIII F8B antibody
    • Factor VIIIa light chain antibody
    • FactorVIII antibody
    • FVIII antibody
    • Hema antibody
    • Hemophilia A antibody
    • Hemophilia, classic antibody
    • OTTHUMP00000061446 antibody
    • OTTHUMP00000196174 antibody
    • Procoagulant component antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human skeletal muscle and capillaries labeling Factor VIII with ab139391 at 5 µg/ml.

References

This product has been referenced in:
  • Yang J  et al. Case report of ocular Kaposi's sarcoma. BMC Ophthalmol 17:143 (2017). Read more (PubMed: 28806949) »
  • Wang Q  et al. Method for in vitro differentiation of bone marrow mesenchymal stem cells into endothelial progenitor cells and vascular endothelial cells. Mol Med Rep 14:5551-5555 (2016). ICC/IF ; Dog, Human . Read more (PubMed: 27878275) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Sample
Pig Tissue sections (Optic nerve)
Specification
Optic nerve
Permeabilization
Yes - 3% hydrogen peroxide
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 05 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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