Product nameAnti-Factor VIII antibody
See all Factor VIII primary antibodies
DescriptionMouse polyclonal to Factor VIII
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog
Full length protein corresponding to Human Factor VIII aa 1-216. (Isoform 2) NM_019863.2
MRIQDPGKVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMEL MGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRS NAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQ DGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQ IALRMEVLGCEAQDLY
Database link: P00451-2
- Factor VIII-transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab171825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa.|
FunctionFactor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Involvement in diseaseDefects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
Sequence similaritiesBelongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains.
DomainDomain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
modificationsSulfation on Tyr-1699 is essential for binding vWF.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- AHF antibody
- Antihemophilic factor antibody
- Coagulation factor VIII antibody
All lanes : Anti-Factor VIII antibody (ab171825) at 1 µg/ml
Lane 1 : Factor VIII (isoform 2)-transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
ab171825 has not yet been referenced specifically in any publications.