Key features and details
- Rabbit polyclonal to Factor VIII
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Factor VIII antibody
See all Factor VIII primary antibodies
DescriptionRabbit polyclonal to Factor VIII
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Factor VIII aa 1-216. Isoform 2
MRIQDPGKVFFGNVDSSGIKHNIFNPPIIARYIRLHPTHYSIRSTLRMEL MGCDLNSCSMPLGMESKAISDAQITASSYFTNMFATWSPSKARLHLQGRS NAWRPQVNNPKEWLQVDFQKTMKVTGVTTQGVKSLLTSMYVKEFLISSSQ DGHQWTLFFQNGKVKVFQGNQDSFTPVVNSLDPPLLTRYLRIHPQSWVHQ IALRMEVLGCEAQDLY
Database link: P00451
- IHC-P: Human placenta and renal tissue. ICC/IF: HeLa cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionFactor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Involvement in diseaseDefects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
Sequence similaritiesBelongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains.
DomainDomain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
modificationsSulfation on Tyr-1699 is essential for binding vWF.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- AHF antibody
- Antihemophilic factor antibody
- Coagulation factor VIII antibody
Paraffin-embedded human placental tissue stained for Factor VIII using ab236284 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human renal tissue stained for Factor VIII using ab236284 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Factor VIII using ab236284 at 1/100 dilution in ICC/IF, followed by an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236284 has been referenced in 2 publications.
- Lou L et al. Lycium barbarum polysaccharide induced apoptosis and inhibited proliferation in infantile hemangioma endothelial cells via down-regulation of PI3K/AKT signaling pathway. Biosci Rep 39:N/A (2019). PubMed: 31383785
- Zhao G et al. Clinical diagnosis of adult patients with acute megakaryocytic leukemia. Oncol Lett 16:6988-6997 (2018). PubMed: 30546432