For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    factor-viiia-activity-assay-kit-fluorometric-ab204696.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Blood Coagulation Intrinsic
Share by email

Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)

  • Datasheet
  • SDS
  • Protocol Booklet
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)
  • Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)

Key features and details

  • Assay type: Enzyme activity
  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Plasma, Purified protein, Serum
  • Sensitivity: 1 ng/well

You may also be interested in

ELISA
Product image
Human Tissue Type Plasminogen Activator ELISA Kit (TPA) (ab108914)
Protein
Product image
Recombinant human PD-L1 protein (Active) (ab167713)
ELISA
Product image
Human FVIII ELISA kit (total FVIII antigen) (ab272771)

View more associated products

Overview

  • Product name

    Factor VIIIa Activity Assay Kit (Fluorometric)
    See all Factor VIII kits
  • Detection method

    Fluorescent
  • Sample type

    Serum, Plasma, Purified protein
  • Assay type

    Enzyme activity
  • Sensitivity

    1 ng/well
  • Product overview

    Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696) is based on the ability of FVIIIa to generate FXa. The generated FXa proteolytically cleaves a synthetic substrate and releases a fluorophore, AMC, which can be easily quantified by fluorescence microplate reader.


    The assay is simple, rapid and can detect activity as low as 1 ng of FVIIIa in a variety of samples.

  • Notes

    This product is manufactured by BioVision, an Abcam company and was previously called K358 Factor VIIIa Activity Assay Kit (Fluorometric). K358-100 is the same size as the 100 test size of ab204696.

    The coagulation Factor VIII (anti-hemophilic factor, AHF) is a vital blood-clotting protein. Factor VIII circulates in the bloodstream as an inactive protein, bound to a large multimeric glycoprotein called von Willebrand factor (VWF). Upon an injury to the blood vessel, VWF dissociates from FVIII and releases the active form of FVIII (FVIIIa). In the presence of calcium ions and negatively charged membrane phospholipids, activated factor VIII (FVIIIa) then binds to the activated Factor IX (FIXa) and proteolytically activates factor X (FX) to factor Xa (FXa).

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Enzyme Mix I 1 vial
    Enzyme Mix II 1 vial
    FVIIIa Assay Buffer 1 x 15ml
    FVIIIa Enzyme Standard 1 x 2.6µg
    FXa Substrate-AMC 1 x 200µl
    Phospholipids 1 x 600µl
  • Research areas

    • Cardiovascular
    • Blood
    • Coagulation
    • Intrinsic
  • Function

    Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
  • Involvement in disease

    Defects in F8 are the cause of hemophilia A (HEMA) [MIM:306700]. A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Note=Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non-functional; i.e., the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein.
  • Sequence similarities

    Belongs to the multicopper oxidase family.
    Contains 3 F5/8 type A domains.
    Contains 2 F5/8 type C domains.
    Contains 6 plastocyanin-like domains.
  • Domain

    Domain F5/8 type C 2 is responsible for phospholipid-binding and essential for factor VIII activity.
  • Post-translational
    modifications

    Sulfation on Tyr-1699 is essential for binding vWF.
  • Cellular localization

    Secreted > extracellular space.
  • Target information above from: UniProt accession P00451 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • AHF
    • Antihemophilic factor
    • Coagulation factor VIII
    • coagulation factor VIII, procoagulant component
    • coagulation factor VIIIc
    • DXS1253E
    • F8
    • F8b
    • F8c
    • FA8_HUMAN
    • factor VIII F8B
    • Factor VIIIa light chain
    • FactorVIII
    • FVIII
    • Hema
    • Hemophilia A
    • Hemophilia, classic
    • OTTHUMP00000061446
    • OTTHUMP00000196174
    • Procoagulant component
    see all

Images

  • Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)
    Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)

    Typical Factor VIIIa Standard Curve using Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696).

  • Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)
    Factor VIIIa Activity Assay Kit (Fluorometric) (ab204696)

    Factor VIIIa activity was measured in serum samples in the presence and absence of the master mix. S: Substrate.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab204696? Please let us know so that we can cite the reference in this datasheet.

ab204696 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab204696.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.