Recombinant Anti-Factor X antibody [EPR16249] (ab196023)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16249] to Factor X
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Factor X antibody [EPR16249]
See all Factor X primary antibodies -
Description
Rabbit monoclonal [EPR16249] to Factor X -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Factor X aa 150-300. The exact sequence is proprietary.
Database link: P00742 -
Positive control
- Human fetal liver, Human blood, Human plasma and HepG2 lysates; Human liver tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR16249 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab196023 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/20000. Detects a band of approximately 74 kDa (predicted molecular weight: 54 kDa). | |
IHC-P | 1/1200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. -
Tissue specificity
Plasma; synthesized in the liver. -
Involvement in disease
Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 2159 Human
- Omim: 227600 Human
- SwissProt: P00742 Human
- Unigene: 361463 Human
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Form
Cleaved into the following 3 chains: 1. Factor X light chain 2. Factor X heavy chain 3. Activated factor Xa heavy chain -
Alternative names
- Activated factor Xa heavy chain antibody
- Coagulation factor X antibody
- F10 antibody
see all
Images
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All lanes : Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human blood lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
We hypothesis the 74 kDa band is the precursor of Factor X.
Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor X antibody [EPR16249] (ab196023)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Factor X with ab196023 at 1/1200 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining on Human liver tissue is observed. Counter stained with Hematoxylin.
Secondary control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + Human plasma lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
We hypothesis the 74 kDa band is the precursor of Factor X.
Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479. -
Anti-Factor X antibody [EPR16249] (ab196023) at 1/20000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 74 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
We hypothesis the 74 kDa band is the precursor of Factor X.
Factor X contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This is consistent with what has been seen in PMID: 19691479.
Protocols
Datasheets and documents
Certificate of Compliance
References (0)
ab196023 has not yet been referenced specifically in any publications.