Product nameFactor Xa Activity Assay Kit (Fluorometric)
See all Factor Xa kits
Sample typePlasma, Purified protein
Assay typeEnzyme activity
Sensitivity< 1 ng
Assay time0h 40m
Factor Xa Activity Assay Kit (Fluorometric) (ab204711) utilizes the ability of Factor Xa to cleave a synthetic substrate thereby releasing a fluorophore, AMC, which can be quantified by fluorescence readers. This assay kit is simple, rapid and can detect Factor Xa activity as low as 1 ng.
Factor Xa activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with microplate reader for 30-60 min every 2-3 min
Factor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.22.214.171.124). Factor X, a serine endopeptidase plays an important role at several stages of the coagulation pathway. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor antithrombin to inactivate several coagulation factors including factor Xa.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests FXa Assay Buffer 1 x 15ml FXa Dilution Buffer 1 x 1ml FXa Enzyme Standard 1 x 5µl FXa Substrate 1 x 200µl
FunctionFactor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
Tissue specificityPlasma; synthesized in the liver.
Involvement in diseaseDefects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
Sequence similaritiesBelongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain.
modificationsThe vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Information by UniProt
- Activated factor Xa heavy chain
ab204711 has not yet been referenced specifically in any publications.