Overview

  • Product name

    Factor Xa Assay Kit
    See all Factor Xa kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Quantitative
  • Sensitivity

    0.028 mIU/ml
  • Range

    0.031 mIU/ml - 2 mIU/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Factor Xa Assay Kit (ab154128) is developed to determine Factor Xa activity in human plasma, serum and cell culture samples. The assay couples immunofunctional and direct amidolytic function. A polyclonal antibody specific for human Factor Xa has been pre-coated onto a microplate and Factor Xa is bound to the immobilized antibody. The amidolytic activity of the Factor Xa is quantitated by using a highly specific Factor Xa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the Factor Xa enzymatic activity.

  • Notes

    Factor X (FX) is a plasma serine protease zymogen involved in the blood coagulation cascade. FX is purified from plasma as a two-chain protein consisting of a 45-kDa heavy chain and a 17-kDa light chain. The FX heavy chain in cleaved during coagulation by several different proteases, including the intrinsic Xase complex, the FX-activating enzyme from Russell's viper venom (RVV) and trypsin, and also by the extrinsic (tissue factor/factor VIIa) pathway, to give an active enzyme FXa. FXa, as the activator of prothrombin, occupies a central position linking the two blood coagulation pathways.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Please refer to protocols.
  • Components 1 x 96 tests
    EIA Diluent Concentrate (10x) 1 x 20ml
    FXa Substrate 2 vials
    Human FXa Microplate 1 unit
    Human FXa Standard 1 x 2 vials
    Sealing Tapes 3 units
    Wash Buffer Concentrate (20x) 1 x 30ml
  • Function

    Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.
  • Tissue specificity

    Plasma; synthesized in the liver.
  • Involvement in disease

    Defects in F10 are the cause of factor X deficiency (FA10D) [MIM:227600]. A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis.
  • Sequence similarities

    Belongs to the peptidase S1 family.
    Contains 2 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 1 peptidase S1 domain.
  • Post-translational
    modifications

    The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
    N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.
    The activation peptide is cleaved by factor IXa (in the intrinsic pathway), or by factor VIIa (in the extrinsic pathway).
    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • Activated factor Xa heavy chain
    • F10
    • FA10_HUMAN
    • Stuart factor
    • Stuart-Prower factor
    see all
  • Database links

Images

Protocols

References

ab154128 has not yet been referenced specifically in any publications.

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