Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Product nameFactor Xa Inhibitor Screening Assay Kit (Fluorometric)
Sample typeInhibitor compounds
Assay typeEnzyme activity
Factor Xa Inhibitor Screening Assay Kit (Fluorometric) (ab204712) is based on the ability of Factor Xa to cleave a synthetic substrate thereby releasing a fluorophore, AMC, which can be quantified by a fluorescence reader at Ex/Em = 350/450 nm. In the presence of an inhibitor, the extent of the cleavage reaction is reduced or completely abolished. The loss in the fluorescence intensity can be correlated to the amount of inhibitor present in the assay solution. The Factor Xa Inhibitor Screening Kit is a simple, straightforward, high-throughput assay to screen Factor Xa inhibitors.
Factor Xa (FXa) is the activated form of the coagulation factor X (Stuart-Power factor, thrombokinase, prothrombinase, thromboplastin, E.C.188.8.131.52). Factor X, a serine endopeptidase, plays an important role at several stages of the coagulation pathway. It acts by converting prothrombin into active thrombin by complexing with activated co-factor V in the prothrombinase complex. Unfractionated heparin and various low molecular weight heparins bind to plasma cofactor Antithrombin to inactivate several coagulation factors including factor Xa.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests FXa Assay Buffer 1 x 15ml FXa Dilution Buffer 1 x 1ml FXa Enzyme 1 x 5µl FXa Inhibitor (GGACK Dihydrochloride, 10 mM) 1 x 10µl FXa Substrate 1 x 200µl
ab204712 has not yet been referenced specifically in any publications.