Key features and details
- Rabbit polyclonal to Factor XIa
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Factor XIa antibody
See all Factor XIa primary antibodies
DescriptionRabbit polyclonal to Factor XIa
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Recombinant fragment corresponding to Rat Factor XIa aa 275-516. N-terminal His-tag and GST-tag. Expressed in E. coli.
IPVFCHPNFYNDTDFLGEELDIVDVKGKESCQKMCSDNVRCQFFTYYPSR GSCSERNMHNQNQAQGVRRSCVCSRRVAMAGDPAHHPGTPVWRLHHWKPV DIDSGSLFLWVSINDIRLGSSSPSTETPKTLRVYGGIVNQSEINEDTTFF RVQEMIIHDQYTSAESGFDIALLKLEPAMNYTDEVQSTLQKAKVPLVSNE ECQTRYRKHKITNKVICAGYKEGGKDTCKGDSGGPLSCKHNG
Database link: Q6TUF8
- WB: Recombinant rat Factor XIa protein and HepG2 whole cell lysate (ab7900). IHC-P: Rat liver tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 63 kDa.|
FunctionFactor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
Tissue specificityIsoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
Involvement in diseaseDefects in F11 are the cause of factor XI deficiency (FA11D) [MIM:612416]; also known as plasma thromboplastin antecedent deficiency or Rosenthal syndrome. It is a hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate.
Sequence similaritiesBelongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain.
modificationsActivated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
- Information by UniProt
- coagulation factor XI antibody
- Coagulation factor XIa light chain antibody
- F11 antibody
Formalin-fixed, paraffin-embedded rat liver tissue stained for Factor XIa using ab232726 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-Factor XIa antibody (ab232726) at 2 µg/ml + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Predicted band size: 63 kDa
Anti-Factor XIa antibody (ab232726) at 2 µg/ml + Recombinant rat Factor XIa protein
Predicted band size: 63 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232726 has not yet been referenced specifically in any publications.