RecombinantRabMAb

Recombinant Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

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Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
  • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
  • Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22292-127] to Factor XII - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free
    See all Factor XII primary antibodies

  • Description

    Rabbit monoclonal [EPR22292-127] to Factor XII - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Factor XII aa 1-400. The exact sequence is proprietary.
    Database link: P00748

  • Positive control

    • IP: Human plasma and serum.

  • General notes

    Ab242152 is the carrier-free version of ab242123. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab242152 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22292-127

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 80, 50 kDa (predicted molecular weight: 67 kDa).
IP Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.

    • Target

    • Function

      Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.

    • Involvement in disease

      Defects in F12 are the cause of factor XII deficiency (FA12D) [MIM:234000]; also known as Hageman factor deficiency. This trait is an asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. F12 deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).
      Defects in F12 are the cause of hereditary angioedema type 3 (HAE3) [MIM:610618]; also known as estrogen-related HAE or hereditary angioneurotic edema with normal C1 inhibitor concentration and function. HAE is characterized by episodic local subcutaneous edema, and submucosal edema involving the upper respiratory and gastrointestinal tracts. HAE3 occurs exclusively in women and is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives). It differs from HAE types 1 and 2 in that both concentration and function of C1 inhibitor are normal.

    • Sequence similarities

      Belongs to the peptidase S1 family.
      Contains 2 EGF-like domains.
      Contains 1 fibronectin type-I domain.
      Contains 1 fibronectin type-II domain.
      Contains 1 kringle domain.
      Contains 1 peptidase S1 domain.

    • Post-translational
      modifications

      Factor XII is activated by kallikrein in alpha-factor XIIa, which is then further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of the NH2-terminal heavy chain (Coagulation factor XIIa heavy chain) and the COOH-terminal light chain (Coagulation factor XIIa light chain), connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, a light chain (Beta-factor XIIa part 2), corresponding to the COOH-terminal light chain (Coagulation factor XIIa light chain) and a nonapeptide (Beta-factor XIIa part 1).
      O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Factor XII antibody
        • Beta factor XIIa part 1 antibody
        • Beta factor XIIa part 2 antibody
        • Coagulation factor XII antibody
        • Coagulation factor XIIa heavy chain antibody
        • Coagulation factor XIIa light chain antibody
        • F12 antibody
        • F12 deficiency antibody
        • FA12_HUMAN antibody
        • Factor XII deficiency antibody
        • HAE3 antibody
        • HAEX antibody
        • HAF antibody
        • HAF deficiency antibody
        • Hageman factor antibody
        see all

      Images

      • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
        Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

        Factor XII was immunoprecipitated from 0.35 mg of human serum with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

        Lane 1: Human serum 10 µg (Input). 

        Lane 2: ab242123 IP in human serum. 

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242123 in human serum.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 5 seconds.

        Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID: 8049433, PMID 2473984).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242123).

      • Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
        Immunoprecipitation - Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

        Factor XII was immunoprecipitated from 0.35 mg of human plasma with ab242123 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242123 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

        Lane 1: Human plasma 10 µg (Input). 

        Lane 2: ab242123 IP in human plasma. 

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab242123 in human plasma.

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 5 seconds.

        Factor XII is an 80kDa glycoprotein undergoes catalysis and forms the 70kDa cleavage and 50kDa heavy chain of alpha-factor XIIa. (PMID: 8049433, PMID 2473984).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242123).

      • Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)
        Anti-Factor XII antibody [EPR22292-127] - BSA and Azide free (ab242152)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab242152? Please let us know so that we can cite the reference in this datasheet.

      ab242152 has not yet been referenced specifically in any publications.

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