Overview

  • Product name
    Anti-Factor XIIIa antibody
    See all Factor XIIIa primary antibodies
  • Description
    Rabbit polyclonal to Factor XIIIa
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein corresponding to subunit-A of coagulation Factor XIIIa.

  • Positive control
    • Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab4057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 30 minuts at room temperature (ABC method).
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • Involvement in disease
    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • Sequence similarities
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications
    The activation peptide is released by thrombin.
  • Cellular localization
    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
    • Coagulation factor XIII A chain antibody
    • Coagulation factor XIII A1 polypeptide antibody
    • Coagulation factor XIII A1 subunit antibody
    • Coagulation factor XIII, A polypeptide antibody
    • Coagulation factor XIIIa antibody
    • F13A antibody
    • F13A_HUMAN antibody
    • F13a1 antibody
    • Factor XIIIA antibody
    • Fibrin stabilizing factor, A subunit antibody
    • Fibrinoligase antibody
    • FSF, A subunit antibody
    • Protein glutamine gamma glutamyltransferase A chain antibody
    • Protein-glutamine gamma-glutamyltransferase A chain antibody
    • TGase antibody
    • Transglutaminase A chain antibody
    • Transglutaminase, plasma antibody
    • Transglutaminase. plasma antibody
    see all

Images

  • ab4057 staining formalin fixed paraffin-embedded human bowel tumour tissue sections.  The sample was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% srum for 1 hour at 24°C, prior to incubation with the primary antibody (diluted 1/100 in milk) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab4057.

References

ab4057 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Acetone
Permeabilization
Yes - 0,05% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 24 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bowel tumor)
Specification
bowel tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrat puffer ph 6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 08 2008

Answer

Thank you for your enquiry. Both proteins are involved in the coagulation cascade. Whether you can use the antibodies interchangeably depends on the experiments you are doing. Unfortunately we aren't experts in the field, so we couldn't tell you which one would be better to use in your particular situation. I am not sure why placenta is suggested as the positive control. It is possible that the protein shows higher expression in placental blood vessels than vessels in other tissues. If you would like me to enquire further about this matter, I would be happy to. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Answer

Thank you for your enquiry. Factor XIIIa is a coagulation protein present in the plasma. Staining is cytoplasmic. It should stain the endothelial cells of the blood vessels. It appears that the datasheet states that the cellular localization is cytoplasmic and secreted, not cytoplasmic and nuclear. I completely missed that, and I apologize. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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Answer

Thank you for your response. This antibody has been tested for IHC using human placenta so it would be very important to run an experiment with tested positive control alongside with the samples. As you have mentioned in your e-mail, dendritic cells also express Factor XIIIa. However, the expressed levels of Factor XIIIa may be different from that in the placenta which usually shows very high levels of this protein. We have looked through our order record and actually the batch number you received was 77421. We have sold many vials form this batch without any problem. It may well be that the vial you received has become inactivated during shipping and/or storage. We can offer you a new vial free of charge. Please do let us know if you are interested in, and then we can place a new order for you as soon as possible.

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Question
Answer

Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab4057. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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