Overview

  • Product name
    Anti-Factor XIIIa antibody
    See all Factor XIIIa primary antibodies
  • Description
    Rabbit polyclonal to Factor XIIIa
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein corresponding to subunit-A of coagulation Factor XIIIa.

  • Positive control
    • Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab4057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 30 minuts at room temperature (ABC method).
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • Involvement in disease
    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • Sequence similarities
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications
    The activation peptide is released by thrombin.
  • Cellular localization
    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
    • Coagulation factor XIII A chain antibody
    • Coagulation factor XIII A1 polypeptide antibody
    • Coagulation factor XIII A1 subunit antibody
    • Coagulation factor XIII, A polypeptide antibody
    • Coagulation factor XIIIa antibody
    • F13A antibody
    • F13A_HUMAN antibody
    • F13a1 antibody
    • Factor XIIIA antibody
    • Fibrin stabilizing factor, A subunit antibody
    • Fibrinoligase antibody
    • FSF, A subunit antibody
    • Protein glutamine gamma glutamyltransferase A chain antibody
    • Protein-glutamine gamma-glutamyltransferase A chain antibody
    • TGase antibody
    • Transglutaminase A chain antibody
    • Transglutaminase, plasma antibody
    • Transglutaminase. plasma antibody
    see all

Images

  • ab4057 staining formalin fixed paraffin-embedded human bowel tumour tissue sections.  The sample was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% srum for 1 hour at 24°C, prior to incubation with the primary antibody (diluted 1/100 in milk) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab4057.

References

ab4057 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Acetone
Permeabilization
Yes - 0,05% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 24 2009

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bowel tumor)
Specification
bowel tumor
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrat puffer ph 6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Feb 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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