Immunohistochemistry (Frozen sections) abreview for Anti-Factor XIIIa antibody

Good
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Acetone
Permeabilization
Yes - 0,05% Triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/25
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/Milk 5%/0,05% Triton

Secondary antibody

Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/100

Additional data

Additional Notes
an ABC method has been used to enhance the staining

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Submitted Apr 24 2009

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