Anti-Factor XIIIa antibody (ab97636)
Key features and details
- Rabbit polyclonal to Factor XIIIa
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Factor XIIIa antibody
See all Factor XIIIa primary antibodies -
Description
Rabbit polyclonal to Factor XIIIa -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 228 of Human Factor XIIIa (NP_000120)
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Positive control
- H1299 whole cell lysate. Paraffin-embedded H520 xenograft. Paraformaldehyde-fixed A549 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab97636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 83 kDa. | |
| IHC-P | 1/500 - 1/3000. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
Involvement in disease
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsThe activation peptide is released by thrombin. -
Cellular localization
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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Database links
- Entrez Gene: 2162 Human
- Entrez Gene: 74145 Mouse
- Omim: 134570 Human
- SwissProt: P00488 Human
- SwissProt: Q8BH61 Mouse
- Unigene: 335513 Human
- Unigene: 235105 Mouse
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Alternative names
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody (ab97636)Immunohistochemical analysis of paraffin-embedded H520 xenograft, using ab97636 at 1/500 dilution.
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Western blot - Anti-Factor XIIIa antibody (ab97636)Anti-Factor XIIIa antibody (ab97636) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 83 kDa
7.5 % SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Factor XIIIa antibody (ab97636)Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97636 at 1/200 dilution. Lower image is merged with DNA probe.
Protocols
References (0)
ab97636 has not yet been referenced specifically in any publications.
