Anti-Factor XIIIa antibody (ab97636)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Factor XIIIa antibody
See all Factor XIIIa primary antibodies -
Description
Rabbit polyclonal to Factor XIIIa -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 228 of Human Factor XIIIa (NP_000120)
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Positive control
- H1299 whole cell lysate. Paraffin-embedded H520 xenograft. Paraformaldehyde-fixed A549 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab97636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/500. Predicted molecular weight: 83 kDa. | |
| IHC-P | 1/500 - 1/3000. | |
| ICC/IF | 1/100 - 1/200. |
Target
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Function
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
Involvement in disease
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsThe activation peptide is released by thrombin. -
Cellular localization
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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Database links
- Entrez Gene: 2162 Human
- Entrez Gene: 74145 Mouse
- Omim: 134570 Human
- SwissProt: P00488 Human
- SwissProt: Q8BH61 Mouse
- Unigene: 335513 Human
- Unigene: 235105 Mouse
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Alternative names
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody (ab97636)Immunohistochemical analysis of paraffin-embedded H520 xenograft, using ab97636 at 1/500 dilution. -
Western blot - Anti-Factor XIIIa antibody (ab97636)Anti-Factor XIIIa antibody (ab97636) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 83 kDa
7.5 % SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-Factor XIIIa antibody (ab97636)Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97636 at 1/200 dilution. Lower image is merged with DNA probe.
Protocols
References
ab97636 has not yet been referenced specifically in any publications.
