Key features and details
- Rabbit polyclonal to Factor XIIIa
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Factor XIIIa antibody
See all Factor XIIIa primary antibodies
DescriptionRabbit polyclonal to Factor XIIIa
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 228 of Human Factor XIIIa (NP_000120)
- H1299 whole cell lysate. Paraffin-embedded H520 xenograft. Paraformaldehyde-fixed A549 cells.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab97636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 83 kDa.|
|IHC-P||1/500 - 1/3000.|
|ICC/IF||1/100 - 1/200.|
FunctionFactor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Involvement in diseaseDefects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
modificationsThe activation peptide is released by thrombin.
Cellular localizationCytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
- Information by UniProt
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using ab97636 at 1/500 dilution.
Anti-Factor XIIIa antibody (ab97636) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 83 kDa
7.5 % SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97636 at 1/200 dilution. Lower image is merged with DNA probe.
ab97636 has not yet been referenced specifically in any publications.