• Product name
    Anti-Factor XIIIa antibody [AC-1A1]
    See all Factor XIIIa primary antibodies
  • Description
    Mouse monoclonal [AC-1A1] to Factor XIIIa
  • Host species
  • Tested applications
    Suitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    BALB/C mice were injected with recombinant human protein corresponding to A-subunit of coagulation Factor XIII.

  • Positive control
    • Placenta
  • General notes

    This antibody recognizes both the dimer and monomer forms.




Our Abpromise guarantee covers the use of ab1834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method.
WB Use at an assay dependent concentration. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.



  • Function
    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • Involvement in disease
    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • Sequence similarities
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    The activation peptide is released by thrombin.
  • Cellular localization
    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
    • Coagulation factor XIII A chain antibody
    • Coagulation factor XIII A1 polypeptide antibody
    • Coagulation factor XIII A1 subunit antibody
    • Coagulation factor XIII, A polypeptide antibody
    • Coagulation factor XIIIa antibody
    • F13A antibody
    • F13A_HUMAN antibody
    • F13a1 antibody
    • Factor XIIIA antibody
    • Fibrin stabilizing factor, A subunit antibody
    • Fibrinoligase antibody
    • FSF, A subunit antibody
    • Protein glutamine gamma glutamyltransferase A chain antibody
    • Protein-glutamine gamma-glutamyltransferase A chain antibody
    • TGase antibody
    • Transglutaminase A chain antibody
    • Transglutaminase, plasma antibody
    • Transglutaminase. plasma antibody
    see all


  • ab1834 - immunohistochemistry

    Formalin fixed paraffin embedded human placenta stained with Factor XIIIa, using ABC and AEC chromogen.

  • ab1834 staining human placenta by IHC-P.
  • Anti-Factor XIIIa antibody [AC-1A1] (ab1834) at 1/250 dilution + Human placenta tissue lysate - total protein (ab29745) at 10 µg

    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa
    Observed band size: 83 kDa
    Additional bands at: 37 kDa, 74 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 2 minutes
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab1834.
  • Overlay histogram showing A549 cells stained with ab16956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.


This product has been referenced in:
  • Handrkova H  et al. The activation peptide of coagulation factor XIII is vital for its expression and stability. J Thromb Haemost 13:1449-58 (2015). WB, SDS-PAGE . Read more (PubMed: 26083359) »
  • Mitchell JL  et al. Functional factor XIII-A is exposed on the stimulated platelet surface. Blood 124:3982-90 (2014). WB ; Human . Read more (PubMed: 25331118) »

See all 7 Publications for this product

Customer reviews and Q&As

Thank you for your phone call and email.

I apologize as I forget to attach the questionnaire. I have looked into the details and would like to make following suggestions;

ab1834 was able to detect band in Fibrogammin samples so I assu...

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Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

As we discussed on the ph...

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Thank you for your email.

This antibody was used in the following publication:

Ulici V et al. Regulation of gene expression by PI3K in mouse growth plate chondrocytes. PLoS One 5:e8866 (2010). WB, IHC-P; Mouse. (PubMed: 20111593) Read More

Thank you for your reply.

As ab125687 PAI1 antibody [1D5], ab1834 Factor XIIIa antibody [AC-1A1] and ab54488 CD41/Integrin alpha 2b antibody [283.16B7] are all mouse IgG1, Ican suggest to consider the following anti mouse IgG1 secondaries av...

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Thank you for your enquiry. Further to our liveperson discussion yesterday morning I have performed some literature searching and in the following publication factor XIIIA was studied using BOTH reducing and non-reducing conditions. Whilst it is ch...

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Thank you for your enquiry regarding ab1834 and your interest in our products. This particular antibody to Factor XIIIa does not require any antigen retrieval for paraffin sections. Proteinase K is more suitable for in situ hybridization and not for...

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Thank you for your email. I would suggested diluting ab1834 to 1:1000 and optimizing based upon your results. We're unfortunately not aware of any publications which feature the use of this antibody. If you have any additional questions, please do ...

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Thank you for your enquiry. You do not need any pretreatment when staining with this particular antibody. It just works fine. However, if your tissue is over fixed, you need enzymatic treatment.

Recombinant protein was purified using HPLC.

The concentration is 100 ug/ml.

1-10 of 11 Abreviews or Q&A


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