Product nameAnti-Factor XIIIa antibody [AC-1A1]
See all Factor XIIIa primary antibodies
DescriptionMouse monoclonal [AC-1A1] to Factor XIIIa
Tested applicationsSuitable for: ICC/IF, ELISA, IHC-P, IHC-Fr, WB, Flow Cytmore details
Species reactivityReacts with: Mouse, Human
BALB/C mice were injected with recombinant human protein corresponding to A-subunit of coagulation Factor XIII.
This antibody recognizes both the dimer and monomer forms.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Light chain typekappa
Our Abpromise guarantee covers the use of ab1834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionFactor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Involvement in diseaseDefects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
modificationsThe activation peptide is released by thrombin.
Cellular localizationCytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
- Information by UniProt
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
ab1834 - immunohistochemistry
Formalin fixed paraffin embedded human placenta stained with Factor XIIIa, using ABC and AEC chromogen.
ab1834 staining human placenta by IHC-P.
Anti-Factor XIIIa antibody [AC-1A1] (ab1834) at 1/250 dilution + Human placenta tissue lysate - total protein (ab29745) at 10 µg
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 37 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab1834.
Overlay histogram showing A549 cells stained with ab16956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This product has been referenced in:
- Lee JS et al. CD34 stromal expression is inversely proportional to smooth muscle actin expression and extent of morphea. J Eur Acad Dermatol Venereol N/A:N/A (2018). Read more (PubMed: 29888507) »
- Handrkova H et al. The activation peptide of coagulation factor XIII is vital for its expression and stability. J Thromb Haemost 13:1449-58 (2015). WB, SDS-PAGE . Read more (PubMed: 26083359) »