Key features and details
- Rabbit monoclonal [EP3371] to Factor XIIIa
- Suitable for: WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-Factor XIIIa antibody [EP3371]
See all Factor XIIIa primary antibodies
DescriptionRabbit monoclonal [EP3371] to Factor XIIIa
Tested applicationsSuitable for: WBmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Rat, Human
Synthetic peptide within Human Factor XIIIa aa 50-150 (N terminal). The exact sequence is proprietary.
- Human placenta, PMBC and rat platelet lysates
Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab79759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
FunctionFactor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Involvement in diseaseDefects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
modificationsThe activation peptide is released by thrombin.
Cellular localizationCytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
- Information by UniProt
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
All lanes : Anti-Factor XIIIa antibody [EP3371] (ab79759) at 1/2000 dilution
Lane 1 : Human placenta lysate
Lane 2 : PBMC lysate
Lane 3 : Rat platelets lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Additional bands at: 65 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
ab79759 has been referenced in 1 publication.
- Fisher ML et al. Transglutaminase Is Required for Epidermal Squamous Cell Carcinoma Stem Cell Survival. Mol Cancer Res 13:1083-94 (2015). PubMed: 25934691