Recombinant
RabMAb

Recombinant Anti-Factor XIIIa antibody [EP3372] (ab76105)

Overview

  • Product name

    Anti-Factor XIIIa antibody [EP3372]
    See all Factor XIIIa primary antibodies
  • Description

    Rabbit monoclonal [EP3372] to Factor XIIIa
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Factor XIIIa. The exact sequence is proprietary.

  • Positive control

    • WB: mouse plasma, mouse platelet, and PMBC lysates IHC-Fr: mouse colon, and mouse spleen tissues IHC-P: mouse testis, mouse placenta and human skin tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab76105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
WB 1/10000 - 1/100000. Predicted molecular weight: 83 kDa.
IP 1/20.
IHC-P 1/100 - 1/250.

Antigen retrieval is recommended

 

  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
    • Involvement in disease

      Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
    • Sequence similarities

      Belongs to the transglutaminase superfamily. Transglutaminase family.
    • Post-translational
      modifications

      The activation peptide is released by thrombin.
    • Cellular localization

      Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
    • Information by UniProt
    • Database links

    • Alternative names

      • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
      • Coagulation factor XIII A chain antibody
      • Coagulation factor XIII A1 polypeptide antibody
      • Coagulation factor XIII A1 subunit antibody
      • Coagulation factor XIII, A polypeptide antibody
      • Coagulation factor XIIIa antibody
      • F13A antibody
      • F13A_HUMAN antibody
      • F13a1 antibody
      • Factor XIIIA antibody
      • Fibrin stabilizing factor, A subunit antibody
      • Fibrinoligase antibody
      • FSF, A subunit antibody
      • Protein glutamine gamma glutamyltransferase A chain antibody
      • Protein-glutamine gamma-glutamyltransferase A chain antibody
      • TGase antibody
      • Transglutaminase A chain antibody
      • Transglutaminase, plasma antibody
      • Transglutaminase. plasma antibody
      see all

    Images

    • Immunohistochemistry (Frozn sections) analysis of mouse colon tissue sections labeling Factor XIIIa with purified ab76105 at 1:50 (1.3µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    • All lanes : Anti-Factor XIIIa antibody [EP3372] (ab76105) at 0.065 µg/ml

      Lane 1 : Mouse plasma
      Lane 2 : Mouse platelet

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

      Predicted band size: 83 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • Immunohistochemistry (Frozn sections) analysis of mouse spleen tissue sections labeling Factor XIIIa with purified ab76105 at 1:50 (1.3µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Factor XIIIa with purified ab76105 at 1:1000 (0.27 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse placenta tissue sections labeling Factor XIIIa with purified ab76105 at 1:1000 (0.27 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
    • Anti-Factor XIIIa antibody [EP3372] (ab76105) at 1/100000 dilution + PMBC cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 83 kDa
      Observed band size: 83 kDa

    • ab76105, at 1/100 dilution, staining Factor XIIIa in paraffin-embedded human skin tissue by Immunohistochemistry. Note positive staining of dendrocytes within the papillary dermis.

    References

    ab76105 has not yet been referenced specifically in any publications.

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