Product nameAnti-Factor XIIIa antibody [EPR10360]
See all Factor XIIIa primary antibodies
DescriptionRabbit monoclonal [EPR10360] to Factor XIIIa
Tested applicationsSuitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment within Human Factor XIIIa. The exact sequence is proprietary.
Database link: P00488
- Human spleen and Human placenta lysates; Human fibrosarcoma and placenta tissues; U937 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab179444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionFactor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Involvement in diseaseDefects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
modificationsThe activation peptide is released by thrombin.
Cellular localizationCytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
- Information by UniProt
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Factor XIIIa with ab179444 at 1/100.
All lanes : Anti-Factor XIIIa antibody [EPR10360] (ab179444) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 83 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of U937 cells labeling Factor XIIIa with ab179444 at 1/50 (green). DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized U937 cells labeling Factor XIIIa with ab179444 at 1/10 (red) or a rabbit IgG (negative) (green).
Immunohistochemical analysis of paraffin embedded Human fibrosarcoma tissue labeling Factor XIIIa with ab179444 at 1/100.
ab179444 has not yet been referenced specifically in any publications.