RecombinantRabMAb

Recombinant Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
  • Western blot - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
  • Immunocytochemistry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
  • Flow Cytometry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
  • Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10360] to Factor XIIIa - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free
    See all Factor XIIIa primary antibodies

  • Description

    Rabbit monoclonal [EPR10360] to Factor XIIIa - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Recombinant fragment within Human Factor XIIIa. The exact sequence is proprietary.
    Database link: P00488

  • General notes

    Ab250089 is the carrier-free version of ab179444. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250089 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab250089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Function

      Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

    • Involvement in disease

      Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

    • Sequence similarities

      Belongs to the transglutaminase superfamily. Transglutaminase family.

    • Post-translational
      modifications

      The activation peptide is released by thrombin.

    • Cellular localization

      Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
      • Coagulation factor XIII A chain antibody
      • Coagulation factor XIII A1 polypeptide antibody
      • Coagulation factor XIII A1 subunit antibody
      • Coagulation factor XIII, A polypeptide antibody
      • Coagulation factor XIIIa antibody
      • F13A antibody
      • F13A_HUMAN antibody
      • F13a1 antibody
      • Factor XIIIA antibody
      • Fibrin stabilizing factor, A subunit antibody
      • Fibrinoligase antibody
      • FSF, A subunit antibody
      • Protein glutamine gamma glutamyltransferase A chain antibody
      • Protein-glutamine gamma-glutamyltransferase A chain antibody
      • TGase antibody
      • Transglutaminase A chain antibody
      • Transglutaminase, plasma antibody
      • Transglutaminase. plasma antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      This data was developed using ab179444, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human placenta tissue labeling Factor XIIIa with ab179444 at 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Western blot - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      Western blot - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      All lanes : Anti-Factor XIIIa antibody [EPR10360] (ab179444) at 1/1000 dilution

      Lane 1 : Human spleen tissue lysate
      Lane 2 : Human placenta tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated at 1/500 dilution

      Developed using the ECL technique.

      Predicted band size: 83 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab179444, the same antibody clone in a different buffer formulation.

    • Immunocytochemistry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      Immunocytochemistry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      This data was developed using ab179444, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of U937 cells labeling Factor XIIIa with ab179444 at 1/50 (green). DAPI nuclear staining (blue).
    • Flow Cytometry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      Flow Cytometry - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      This data was developed using ab179444, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized U937 cells labeling Factor XIIIa with ab179444 at 1/10 (red) or a rabbit IgG (negative) (green).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      This data was developed using ab179444, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human fibrosarcoma tissue labeling Factor XIIIa with ab179444 at 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    • Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)
      Anti-Factor XIIIa antibody [EPR10360] - BSA and Azide free (ab250089)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab250089? Please let us know so that we can cite the reference in this datasheet.

    ab250089 has not yet been referenced specifically in any publications.

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