Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859)

Overview

  • Product name

    Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free
    See all Factor XIIIa primary antibodies
  • Description

    Mouse monoclonal [F13A1/1448] to Factor XIIIa - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: Protein Array, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Factor XIIIa aa 46-181. The exact sequence is proprietary.
    Database link: P00488

  • Positive control

    • Human histiocytoma tissue; HeLa cell lysate; Human recombinant Factor XIIIa protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab218859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Protein Array Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 83 kDa.
IHC-P Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

(Primary incubation for 30 min at RT).

Target

  • Function

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • Involvement in disease

    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications

    The activation peptide is released by thrombin.
  • Cellular localization

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • Database links

  • Alternative names

    • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
    • Coagulation factor XIII A chain antibody
    • Coagulation factor XIII A1 polypeptide antibody
    • Coagulation factor XIII A1 subunit antibody
    • Coagulation factor XIII, A polypeptide antibody
    • Coagulation factor XIIIa antibody
    • F13A antibody
    • F13A_HUMAN antibody
    • F13a1 antibody
    • Factor XIIIA antibody
    • Fibrin stabilizing factor, A subunit antibody
    • Fibrinoligase antibody
    • FSF, A subunit antibody
    • Protein glutamine gamma glutamyltransferase A chain antibody
    • Protein-glutamine gamma-glutamyltransferase A chain antibody
    • TGase antibody
    • Transglutaminase A chain antibody
    • Transglutaminase, plasma antibody
    • Transglutaminase. plasma antibody
    see all

Images

  • This data was produced with ab218392, the same antibody in a different formulation with BSA and Azide.
    ab218392 was tested in protein array against over 19000 different full-length human proteins.
    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
    A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded human histiocytoma tissue labeling Factor XIIIa with ab218859 at 2 µg/ml.

  • All lanes : Anti-Factor XIIIa antibody [F13A1/1448] - BSA and Azide free (ab218859) at 1 µg/ml

    Lane 1 : Recombinant Human Factor XIIIa protein
    Lane 2 : HeLa cell lysate

    Predicted band size: 83 kDa

References

ab218859 has not yet been referenced specifically in any publications.

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