Overview

  • Product name

    Factor XIIIa Assay Kit
  • Detection method

    Colorimetric
  • Sample type

    Plasma, Purified protein
  • Product overview

    The Factor XIIIa Assay Kit (ab241013) utilizes the transglutaminase activity of factor XIIIa to cross link an amine-containing substrate to glutamine-containing substrate resulting in the loss of ammonia which can be quantitatively measured by a colorimetric assay. The kit is easy-to-use and can detect Factor XIIIa (as low as 0.1 Loewy U) from plasma and purified protein samples.


     

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    FXIIIa Activation Buffer (4X) 1 x 2.5ml
    FXIIIa Assay Buffer 1 x 2.5ml
    FXIIIa Detection Buffer (2X) 1 x 5ml
    FXIIIa Inhibitor (Iodoacetamide) 1 vial
    FXIIIa Probe 1 x 0.2ml
    FXIIIa Reaction Buffer (4X) 1 x 2.5ml
    Human Factor XIIIa (0.5 Loewy U/µl) 1 x 20µl
    NH4Cl Standard (10 mM) 1 x 100µl
  • Research areas

  • Function

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • Involvement in disease

    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • Sequence similarities

    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Post-translational
    modifications

    The activation peptide is released by thrombin.
  • Cellular localization

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • Alternative names

    • bA525O21.1 (coagulation factor XIII, A1 polypeptide)
    • Coagulation factor XIII A chain
    • Coagulation factor XIII A1 polypeptide
    • Coagulation factor XIII A1 subunit
    • Coagulation factor XIII, A polypeptide
    • Coagulation factor XIIIa
    • F13A
    • F13A_HUMAN
    • F13a1
    • Factor XIIIA
    • Fibrin stabilizing factor, A subunit
    • Fibrinoligase
    • FSF, A subunit
    • Protein glutamine gamma glutamyltransferase A chain
    • Protein-glutamine gamma-glutamyltransferase A chain
    • TGase
    • Transglutaminase A chain
    • Transglutaminase, plasma
    • Transglutaminase. plasma
    see all

Images

  • Kinetic progressive curves for different amounts of FXIIIa Enzyme.
  • Kinetic progressive curves for Plasma Samples with and without Iodoacetamide control.
  • Standard curves for NH4Cl (n = 3).
  • Standard curves for NH4Cl was used to estimate FXIIIa activity in plasma samples (n = 3).

Protocols

References

ab241013 has not yet been referenced specifically in any publications.

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