Anti-Factor XIII/XIIIa antibody (ab45000)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-Factor XIII/XIIIa antibody
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DescriptionRabbit polyclonal to Factor XIII/XIIIa
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant full length protein corresponding to A-subunit of human coagulation Factor XIII.
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Positive control
- Capillary hemangioma, dermatofibroma and placenta.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading... -
PurityProtein G purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab45000 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 160 kDa (un-reduced dimer) or 80kDa (reduced monomer). | |
| IHC-P | 1/2000. |
Target
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RelevanceFactor XIII is a beta globulin found in plasma and is composed of two subunits. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Factor XIII A is the catalytic subunit and is a dimmer of molecular weight 160kDa. The B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIII A, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
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Cellular localizationCytoplasmic in most tissues, but also secreted in the blood plasma
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Database links
- Entrez Gene: 2162 Human
- Omim: 134570 Human
- SwissProt: P00488 Human
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Alternative names
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A chain precursor antibody
- Coagulation factor XIII A polypeptide antibody
see all
References
ab45000 has not yet been referenced specifically in any publications.
