Anti-FADD antibody (ab24533)

Submit a review Q&A (2)References (16)
Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody (ab24533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody (ab24533)

Key features and details

  • Rabbit polyclonal to FADD
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab24533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

  • Tissue specificity

    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

  • Sequence similarities

    Contains 1 death domain.
    Contains 1 DED (death effector) domain.

  • Domain

    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt

  • Database links

    • Alternative names

      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody (ab24533)
      Immunocytochemistry/ Immunofluorescence - Anti-FADD antibody (ab24533)
      ICC/IF image of ab24533 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab24533, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody (ab24533)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody (ab24533)
      IHC image of ab24533 staining in human normal lymph node formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24533, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    References (16)

    Publishing research using ab24533? Please let us know so that we can cite the reference in this datasheet.

    ab24533 has been referenced in 16 publications.

    • Zhou J  et al. Herbs-partitioned moxibustion alleviates aberrant intestinal epithelial cell apoptosis by upregulating A20 expression in a mouse model of Crohn's disease. World J Gastroenterol 25:2071-2085 (2019). PubMed: 31114134
    • Liu W  et al. A Novel AKT Activator, SC79, Prevents Acute Hepatic Failure Induced by Fas-Mediated Apoptosis of Hepatocytes. Am J Pathol 188:1171-1182 (2018). PubMed: 29673487
    • Jing ZT  et al. Hepatitis B Virus Surface Antigen Enhances the Sensitivity of Hepatocytes to Fas-Mediated Apoptosis via Suppression of AKT Phosphorylation. J Immunol 201:2303-2314 (2018). PubMed: 30171166
    • Yu R  et al. Kruppel-like factor 4 inhibits non-small cell lung cancer cell growth and aggressiveness by stimulating transforming growth factor-ß1-meidated ERK/JNK/NF-?B signaling pathways. Tumour Biol 39:1010428317705574 (2017). PubMed: 28631556
    • Shin SJ  et al. The traditional Korean herbal medicine Ga-Gam-Nai-Go-Hyan suppresses testosterone-induced benign prostatic hyperplasia by regulating inflammatory responses and apoptosis. Exp Ther Med 13:1025-1031 (2017). WB ; Rat . PubMed: 28450936
    View all Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    I am trying to source an antibody that works against pig FADD. We have already tried the Stressgen antibody raised against the peptide from the human sequence KIDSIEDRYPPNLTER, and it did not work on pig cell lysates (contrary to the data sheet) but worked on human cell lysates. Upon closer examination, I found that the pig sequence is closer to the mouse peptide in this region and there are quite a number of differece between the pig and human sequences. The abcam antibody ab24533 is raised against a peptide that includes this region, but your website only gives a range for the peptide of aa 100-150 and no precise sequence info. Does your peptide include the region ICDNVGKDWRRLARQLKVSD, which is immediately N terminal to the KIDSIEDRYPPNLTER sequence and a perfect match with the pig (Sus scrofa) sequence? I am basically wanting to know whether or not there is a chance that the abcam antibody will work on pig cells. Also, the dog and pig sequences are almost identical. Thank you

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    Answer

    Thank you for your patience in this matter. I have received a reply from the originator of ab24533 (FADD antibody). The immunogenic peptide sequence used in raising this product is: KIDSIEDRYPRNLTERVRESL. Please do not hesitate to contact us again if you have further questions.

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    Question

    What residues does it correspond to on FADD? The product sheet states that it surrounds aa 128 of human FADD. I'm not sure exactly which residues it corresponds to.

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    Answer

    Thank you for your recent enquiry. I have been informed that the immunogen sequence is within the range of amino acids 100-150 of human FADD, I am unable to provide the specific immunogen sequence as this information is confidential. I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

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