Anti-FADD antibody [EPR4415] (ab108601)
- Datasheet
- Specific References (3)
- Protocols
Overview
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Product nameAnti-FADD antibody [EPR4415]
See all FADD primary antibodies -
DescriptionRabbit monoclonal [EPR4415] to FADD
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-P, ICC, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human FADD aa 1-150. The exact sequence is proprietary.
Database link: Q13158 -
Positive control
- A431, Jurkat, HeLa, and SKBR-3 cell lysates, Human kidney tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR4415
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab108601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| ICC | 1/100 - 1/250. | |
| Flow Cyt | Use at an assay dependent concentration. |
Target
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FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
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Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
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Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain. -
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
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Database links
- Entrez Gene: 8772 Human
- Omim: 602457 Human
- SwissProt: Q13158 Human
- Unigene: 86131 Human
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Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Images
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![Western blot - Anti-FADD antibody [EPR4415] (ab108601)](https://www.abcam.com/ps/products/108/ab108601/Images/ab108601-256176-ab108601KOWB.jpg)
Western blot - Anti-FADD antibody [EPR4415] (ab108601)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: FADD knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab108601 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] (ab108601)](https://www.abcam.com/ps/products/108/ab108601/Images/FADD-Primary-antibodies-ab108601-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] (ab108601)Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108601 at a dilution of 1/100. -
![Flow Cytometry - Anti-FADD antibody [EPR4415] (ab108601)](https://www.abcam.com/ps/products/108/ab108601/Images/ab108601-268994-ab108601FC1.jpg)
Flow Cytometry - Anti-FADD antibody [EPR4415] (ab108601)Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling FADD with purified ab108601 at 1/140 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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![Western blot - Anti-FADD antibody [EPR4415] (ab108601)](https://www.abcam.com/ps/products/108/ab108601/Images/FADD-Primary-antibodies-ab108601-2.JPG)
Western blot - Anti-FADD antibody [EPR4415] (ab108601)All lanes : Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : SKBR-3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Protocols
References
This product has been referenced in:
- Ali M & Mocarski ES Proteasome inhibition blocks necroptosis by attenuating death complex aggregation. Cell Death Dis 9:346 (2018). Read more (PubMed: 29497034) »
- Liu Y et al. MiR-7a is an important mediator in Fas-associated protein with death domain (FADD)-regulated expression of focal adhesion kinase (FAK). Oncotarget 7:51393-51407 (2016). WB . Read more (PubMed: 27286445) »
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