Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-FADD antibody [EPR4415] (ab108601)

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Overview

  • Product name

    Anti-FADD antibody [EPR4415]
    See all FADD primary antibodies

  • Description

    Rabbit monoclonal [EPR4415] to FADD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human FADD aa 1-150. The exact sequence is proprietary.
    Database link: Q13158

  • Positive control

    • A431, Jurkat, HeLa, and SKBR-3 cell lysates, Human kidney tissue

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab108601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

  • Tissue specificity

    Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

  • Sequence similarities

    Contains 1 death domain.
    Contains 1 DED (death effector) domain.

  • Domain

    Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
  • Information by UniProt

  • Database links

    • Alternative names

      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all

    Images

    • Western blot - Anti-FADD antibody [EPR4415] (ab108601)
      Western blot - Anti-FADD antibody [EPR4415] (ab108601)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: FADD knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108601 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab108601 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab108601 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] (ab108601)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR4415] (ab108601)

      Immunohistochemical staining of paraffin-embedded Human kidney tissue using ab108601 at a dilution of 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-FADD antibody [EPR4415] (ab108601)
      Flow Cytometry - Anti-FADD antibody [EPR4415] (ab108601)

      Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling FADD with purified ab108601 at 1/140 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Western blot - Anti-FADD antibody [EPR4415] (ab108601)
      Western blot - Anti-FADD antibody [EPR4415] (ab108601)
      All lanes : Anti-FADD antibody [EPR4415] (ab108601) at 1/1000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    References

    This product has been referenced in:

    • Ali M & Mocarski ES Proteasome inhibition blocks necroptosis by attenuating death complex aggregation. Cell Death Dis 9:346 (2018). Read more (PubMed: 29497034) »
    • Liu Y  et al. MiR-7a is an important mediator in Fas-associated protein with death domain (FADD)-regulated expression of focal adhesion kinase (FAK). Oncotarget 7:51393-51407 (2016). WB . Read more (PubMed: 27286445) »
    See all 3 Publications for this product

    Publishing research using ab108601? Please let us know so that we can cite the reference in this datasheet.

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