Recombinant Anti-FADD antibody [EPR5030] (ab124812)
- Datasheet
- References (7)
- Protocols
Overview
-
Product nameAnti-FADD antibody [EPR5030]
See all FADD primary antibodies -
DescriptionRabbit monoclonal [EPR5030] to FADD
-
Host speciesRabbit
-
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC or IP -
Species reactivityReacts with: Mouse
-
Immunogen
Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.
-
Positive control
- Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
-
General notes
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
-
ClonalityMonoclonal
-
Clone numberEPR5030
-
IsotypeIgG
-
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| IHC-Fr | Use at an assay dependent concentration. |
Target
-
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
-
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
-
Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain. -
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
-
Database links
- Entrez Gene: 14082 Mouse
- SwissProt: Q61160 Mouse
- Unigene: 5126 Mouse
-
Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Images
-
![Western blot - Anti-FADD antibody [EPR5030] (ab124812)](https://www.abcam.com/ps/products/124/ab124812/Images/ab124812-138725-anti-fadd-antibody-epr5030-western-blot.jpg)
Western blot - Anti-FADD antibody [EPR5030] (ab124812)All lanes : Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)](https://www.abcam.com/ps/products/124/ab124812/Images/ab124812-138724-anti-fadd-antibody-epr5030-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.
Datasheets and documents
References
This product has been referenced in:
- Wang JB et al. A systems pharmacology-oriented discovery of a new therapeutic use of the TCM formula Liuweiwuling for liver failure. Sci Rep 8:5645 (2018). Read more (PubMed: 29618826) »
- Meng H et al. Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis. Proc Natl Acad Sci U S A 115:E2001-E2009 (2018). Read more (PubMed: 29440439) »
