Recombinant
Recombinant Anti-FADD antibody [EPR5030] (ab124812)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5030] to FADD
- Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr
- Reacts with: Mouse
Overview
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Product name
Anti-FADD antibody [EPR5030]
See all FADD primary antibodies -
Description
Rabbit monoclonal [EPR5030] to FADD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.
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Positive control
- Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
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General notes
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5030 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab124812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. |
Application notes
Is unsuitable for ICC or IP.
Target
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Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. -
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain. -
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor. - Information by UniProt
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Database links
- Entrez Gene: 14082 Mouse
- SwissProt: Q61160 Mouse
- Unigene: 5126 Mouse
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Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Images
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![Western blot - Anti-FADD antibody [EPR5030] (ab124812)](https://www.abcam.com/ps/products/124/ab124812/Images/ab124812-138725-anti-fadd-antibody-epr5030-western-blot.jpg)
Western blot - Anti-FADD antibody [EPR5030] (ab124812)All lanes : Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution
Lane 1 : Mouse spleen lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)](https://www.abcam.com/ps/products/124/ab124812/Images/ab124812-138724-anti-fadd-antibody-epr5030-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
ab124812 has been referenced in 8 publications.
- Cai F et al. ADT-OH, a hydrogen sulfide-releasing donor, induces apoptosis and inhibits the development of melanoma in vivo by upregulating FADD. Cell Death Dis 11:33 (2020). PubMed: 31949127
- Meng H et al. Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis. Proc Natl Acad Sci U S A 115:E2001-E2009 (2018). PubMed: 29440439
- Zhang X et al. Dominant Negative FADD/MORT1 Inhibits the Development of Intestinal Intraepithelial Lymphocytes With a Marked Defect on CD8aa+TCR?d+ T Cells. Front Immunol 9:2038 (2018). PubMed: 30250469
- Wang JB et al. A systems pharmacology-oriented discovery of a new therapeutic use of the TCM formula Liuweiwuling for liver failure. Sci Rep 8:5645 (2018). PubMed: 29618826
- Wang H et al. PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP. Proc Natl Acad Sci U S A 114:11944-11949 (2017). PubMed: 29078411
- Geng J et al. Regulation of RIPK1 activation by TAK1-mediated phosphorylation dictates apoptosis and necroptosis. Nat Commun 8:359 (2017). PubMed: 28842570
- Bowman BM et al. Phosphorylation of FADD by the kinase CK1a promotes KRASG12D-induced lung cancer. Sci Signal 8:ra9 (2015). PubMed: 25628462
- Jaiswal MK et al. Regulation of apoptosis and innate immune stimuli in inflammation-induced preterm labor. J Immunol 191:5702-13 (2013). WB, IHC-Fr ; Mouse . PubMed: 24163412
