Name: Anti-FADD antibody [EPR5030]
Brand: RabMAb
SKU: ab124812
Rating: 4 (9 reviews)

Product name

Anti-FADD antibody [EPR5030]
See all FADD primary antibodies
Description

Rabbit monoclonal [EPR5030] to FADD
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC or IP
Species reactivity

Reacts with: Mouse
Immunogen

Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.
Positive control
- Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
General notes

Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR5030
Isotype

IgG
Research areas

Alternative Versions
- Anti-FADD antibody [EPR5030] (Phycoerythrin) (ab211700)
- Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls

Our Abpromise guarantee covers the use of ab124812 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr		Use at an assay dependent concentration.

Application notes

Is unsuitable for ICC or IP.

Function

Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificity

Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similarities

Contains 1 death domain.
Contains 1 DED (death effector) domain.
Domain

Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
Target information above from: UniProt accession Q13158 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 14082 Mouse
- SwissProt: Q61160 Mouse
- Unigene: 5126 Mouse
Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
- Fas (TNFRSF6) associated via death domain antibody
- Fas associated via death domain antibody
- Fas associating death domain containing protein antibody
- Fas associating protein antibody
- Fas associating protein with death domain antibody
- Fas TNFRSF6 associated via death domain antibody
- FAS-associated death domain protein antibody
- FAS-associating death domain-containing protein antibody
- GIG 3 antibody
- GIG3 antibody
- Growth inhibiting gene 3 protein antibody
- Growth-inhibiting gene 3 protein antibody
- H sapiens mRNA for mediator of receptor induced toxicity antibody
- Mediator of receptor induced toxicity antibody
- MGC8528 antibody
- MORT 1 antibody
- MORT1 antibody
- Protein FADD antibody
see all

Western blot - Anti-FADD antibody [EPR5030] (ab124812)

All lanes : Anti-FADD antibody [EPR5030] (ab124812) at 1/1000 dilution

Lane 1 : Mouse spleen lysate
Lane 2 : Raw264.7 cell lysate
Lane 3 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa
Observed band size: 28 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FADD antibody [EPR5030] (ab124812)

ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Datasheet

This product has been referenced in:

Wang JB et al. A systems pharmacology-oriented discovery of a new therapeutic use of the TCM formula Liuweiwuling for liver failure. Sci Rep 8:5645 (2018). Read more (PubMed: 29618826) »
Wang H et al. PELI1 functions as a dual modulator of necroptosis and apoptosis by regulating ubiquitination of RIPK1 and mRNA levels of c-FLIP. Proc Natl Acad Sci U S A 114:11944-11949 (2017). Read more (PubMed: 29078411) »

See all 5 Publications for this product

Publishing research using ab124812? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-9 of 9 Abreviews or Q&A

Application

Western blot

Sample

Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

0.5 µg

Specification

Rabbit Reticulocyte

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 09 2017

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MOPS buffer))

Loading amount

1e+006 cells

Specification

BHK-21 (Kidney Fibroblasts)

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 08 2017

Application

Western blot

Sample

Chinese Hamster Cell lysate - whole cell (Chinese hamster ovary (CHO) cells)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))

Loading amount

50000 cells

Specification

Chinese hamster ovary (CHO) cells

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 12 2016

Abcam has not validated the combination of species/application used in this Abreview.

Application

Western blot

Sample

Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)

Loading amount

500000 cells

Specification

Canine Kidney Epithelial MDCK cells

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 11 2016

Application

Immunoprecipitation

Sample

Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)

Total protein in input

5e+006 cells

Treatment

50 ng/mL TNFalpha for 5 hours

Immuno-precipitation step

Other - Protein G Dynabeads

Specification

mouse embryonic fibroblasts

Abcam user community

Verified customer

Submitted May 03 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (Human Foreskin Fibroblasts & Mouse embryonic fibro)

Gel Running Conditions

Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)

Loading amount

200000 cells

Specification

Human Foreskin Fibroblasts & Mouse embryonic fibro

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 05 2016

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)

Gel Running Conditions

Reduced Denaturing

Loading amount

10000 cells

Treatment

With or without 100uM zVAD

Specification

mouse embryonic fibroblasts

Blocking step

Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C

Dr. Yi-Chieh Perng

Verified customer

Submitted Nov 30 2015

Application

Western blot

Loading amount

5 µg

Gel Running Conditions

Reduced Denaturing (4-12)

Sample

Mouse Tissue lysate - whole (uterus)

Specification

uterus

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Varkha Agrawal

Verified customer

Submitted Jan 07 2014

Application

Immunohistochemistry (Frozen sections)

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C

Sample

Mouse Tissue sections (Uterus)

Specification

Uterus

Permeabilization

Fixative

Paraformaldehyde

Dr. Mukesh Jaiswal

Verified customer

Submitted Dec 04 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Location selector

Your location is currently set to:

Custom solutions & partnerships

Anti-FADD antibody [EPR5030] (ab124812)

Overview

Properties

Associated products

Applications

Target

Images

Protocols

Datasheets and documents

References

This product has been referenced in:

Customer reviews and Q&As

Western blot abreview for Anti-FADD antibody [EPR5030]

Western blot abreview for Anti-FADD antibody [EPR5030]

Western blot abreview for Anti-FADD antibody [EPR5030]

Western blot abreview for Anti-FADD antibody [EPR5030]

Immunoprecipitation abreview for Anti-FADD antibody [EPR5030]

Western blot abreview for Anti-FADD antibody [EPR5030]

Western blot abreview for Anti-FADD antibody [EPR5030]

Western blot abreview for Anti-FADD antibody [EPR5030]

Immunohistochemistry (Frozen sections) abreview for Anti-FADD antibody [EPR5030]