Recombinant Anti-FADD antibody [EPR5030] - BSA and Azide free (ab229444)


  • Product name

    Anti-FADD antibody [EPR5030] - BSA and Azide free
    See all FADD primary antibodies
  • Description

    Rabbit monoclonal [EPR5030] to FADD - BSA and Azide free
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
    Unsuitable for: ICC or IP
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.

  • Positive control

    • Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab229444 is a PBS-only buffer formulated version of ab124812, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab124812 for information on protocols, dilutions, and image data.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.



Our Abpromise guarantee covers the use of ab229444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.


IHC-Fr Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function

      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificity

      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similarities

      Contains 1 death domain.
      Contains 1 DED (death effector) domain.
    • Domain

      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt
    • Database links

    • Alternative names

      • FADD antibody
      • FADD protein antibody
      • FADD_HUMAN antibody
      • Fas (TNFRSF6) associated via death domain antibody
      • Fas associated via death domain antibody
      • Fas associating death domain containing protein antibody
      • Fas associating protein antibody
      • Fas associating protein with death domain antibody
      • Fas TNFRSF6 associated via death domain antibody
      • FAS-associated death domain protein antibody
      • FAS-associating death domain-containing protein antibody
      • GIG 3 antibody
      • GIG3 antibody
      • Growth inhibiting gene 3 protein antibody
      • Growth-inhibiting gene 3 protein antibody
      • H sapiens mRNA for mediator of receptor induced toxicity antibody
      • Mediator of receptor induced toxicity antibody
      • MGC8528 antibody
      • MORT 1 antibody
      • MORT1 antibody
      • Protein FADD antibody
      see all


    • This IHC data was generated using the same anti-FADD antibody clone, EPR5030, in a different buffer formulation (cat# ab124812).

      ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.


    ab229444 has not yet been referenced specifically in any publications.

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