Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5030] to FADD - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt, IHC-Fr
- Reacts with: Mouse
Product nameAnti-FADD antibody [EPR5030] - BSA and Azide free
See all FADD primary antibodies
DescriptionRabbit monoclonal [EPR5030] to FADD - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, IHC-Frmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to residues in the C-terminus of Mouse FADD.
- Mouse kidney tissue; Mouse spleen lysate, Raw264.7 cell lysate and NIH/3T3 cell lysate.
ab229444 is the carrier-free version of ab124812 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab229444 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab229444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain.
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
This IHC data was generated using the same anti-FADD antibody clone, EPR5030, in a different buffer formulation (cat# ab124812).
ab124812, at 1/50 dilution, staining FADD in paraffin-embedded Mouse Kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229444 has not yet been referenced specifically in any publications.