Product nameAnti-FADD antibody [OTI1C11]
See all FADD primary antibodies
DescriptionMouse monoclonal [OTI1C11] to FADD
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human FADD. Protein produced in HEK293T cells
Database link: NP_003815
- WB:HEK293T cells transfected with pCMV6-ENTRY FADD cDNA, A431, HepG2, HeLe, A549, MCF7 and Jurkat cell lysates FC: HEK293T cells transfected with a FADD overexpression plasmid
The clone number has been updated from 1C11 to OTI1C11, both clone numbers name the same clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab119059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 23 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain.
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: FADD knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab119059 observed at 25 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab119059 was shown to specifically react with FADD when FADD knockout samples were used. Wild-type and FADD knockout samples were subjected to SDS-PAGE. ab119059 and ab181602 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY FADD cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 23 kDa
All lanes : Anti-FADD antibody [OTI1C11] (ab119059) at 1/200 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
HEK293T cells transfected with either a FADD overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab119059 at 1/100 dilution and then analyzed by flow cytometry.
ab119059 has not yet been referenced specifically in any publications.