Product nameAnti-FADD (phospho S194) antibody
See all FADD primary antibodies
DescriptionRabbit polyclonal to FADD (phospho S194)
SpecificityThis antibody detects endogenous levels of FADD only when phosphorylated at serine 194.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human FADD around the phosphorylation site of serine 194 (A-M-SP-P-M).
- Extracts from HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab55334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionApoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
Tissue specificityExpressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
Sequence similaritiesContains 1 death domain.
Contains 1 DED (death effector) domain.
DomainContains a death domain involved in the binding of the corresponding domain within Fas receptor.
- Information by UniProt
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Paclitaxel (1µM, 60mins).
Lane 2 : Extracts from HeLa cells treated
with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.
Predicted band size: 23 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
ab55334 has not yet been referenced specifically in any publications.