Anti-FADD (phospho S194) antibody (ab55334)
Key features and details
- Rabbit polyclonal to FADD (phospho S194)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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Overview
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Product name
Anti-FADD (phospho S194) antibody
See all FADD primary antibodies -
Description
Rabbit polyclonal to FADD (phospho S194) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human FADD aa 150-250 (phospho S194).
Database link: Q13158 -
Positive control
- Extracts from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab55334 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (5) |
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
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| Notes |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. -
Tissue specificity
Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. -
Sequence similarities
Contains 1 death domain.
Contains 1 DED (death effector) domain. -
Domain
Contains a death domain involved in the binding of the corresponding domain within Fas receptor. - Information by UniProt
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Database links
- Entrez Gene: 8772 Human
- Entrez Gene: 14082 Mouse
- Omim: 602457 Human
- SwissProt: Q13158 Human
- SwissProt: Q61160 Mouse
- Unigene: 86131 Human
- Unigene: 5126 Mouse
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Alternative names
- FADD antibody
- FADD protein antibody
- FADD_HUMAN antibody
see all
Images
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Western blot - Anti-FADD (phospho S194) antibody (ab55334)All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution
Lane 1 : Extracts from HeLa cells treated
with Paclitaxel (1µM, 60mins).
Lane 2 : Extracts from HeLa cells treated
with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.
Predicted band size: 23 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (0)
ab55334 has not yet been referenced specifically in any publications.
