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    fadd-phospho-s194-antibody-ab55334.pdf

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Cell Biology Apoptosis Intracellular Associated Proteins
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Anti-FADD (phospho S194) antibody (ab55334)

  • Datasheet
Reviews (5)Q&A (2)

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Western blot - Anti-FADD (phospho S194) antibody (ab55334)

    Key features and details

    • Rabbit polyclonal to FADD (phospho S194)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-FADD (phospho S194) antibody [EPR1816Y] (ab68475)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-FADD (phospho S194) antibody
      See all FADD primary antibodies
    • Description

      Rabbit polyclonal to FADD (phospho S194)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide corresponding to Human FADD aa 150-250 (phospho S194).
      Database link: Q13158

    • Positive control

      • Extracts from HeLa cells.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Intracellular
      • Associated Proteins
      • Cancer
      • Invasion/microenvironment
      • Apoptosis
      • Death receptors & ligands
      • FADD
      • Cancer
      • Cell Death
      • Apoptosis
      • Receptors
      • Death receptors & ligands
      • FADD
      • Cancer
      • Cell Death
      • Necroptosis

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human FADD protein (ab89242)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab55334 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB (5)
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 23 kDa).

    Target

    • Function

      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
    • Tissue specificity

      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
    • Sequence similarities

      Contains 1 death domain.
      Contains 1 DED (death effector) domain.
    • Domain

      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Target information above from: UniProt accession Q13158 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 8772 Human
      • Entrez Gene: 14082 Mouse
      • Omim: 602457 Human
      • SwissProt: Q13158 Human
      • SwissProt: Q61160 Mouse
      • Unigene: 86131 Human
      • Unigene: 5126 Mouse
      • Alternative names

        • FADD antibody
        • FADD protein antibody
        • FADD_HUMAN antibody
        • Fas (TNFRSF6) associated via death domain antibody
        • Fas associated via death domain antibody
        • Fas associating death domain containing protein antibody
        • Fas associating protein antibody
        • Fas associating protein with death domain antibody
        • Fas TNFRSF6 associated via death domain antibody
        • FAS-associated death domain protein antibody
        • FAS-associating death domain-containing protein antibody
        • GIG 3 antibody
        • GIG3 antibody
        • Growth inhibiting gene 3 protein antibody
        • Growth-inhibiting gene 3 protein antibody
        • H sapiens mRNA for mediator of receptor induced toxicity antibody
        • Mediator of receptor induced toxicity antibody
        • MGC8528 antibody
        • MORT 1 antibody
        • MORT1 antibody
        • Protein FADD antibody
        see all

      Images

      • Western blot - Anti-FADD (phospho S194) antibody (ab55334)
        Western blot - Anti-FADD (phospho S194) antibody (ab55334)
        All lanes : Anti-FADD (phospho S194) antibody (ab55334) at 1/500 dilution

        Lane 1 : Extracts from HeLa cells treated
        with Paclitaxel (1µM, 60mins).
        Lane 2 : Extracts from HeLa cells treated
        with Paclitaxel (1µM, 60mins), and with the immunising phosphpeptide.

        Predicted band size: 23 kDa
        Observed band size: 30 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (0)

      Publishing research using ab55334? Please let us know so that we can cite the reference in this datasheet.

      ab55334 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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      1-5 of 5 Abreviews

      Western blot abreview for Anti-FADD (phospho S194) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel (MES buffer))
      Loading amount
      0.5 µg
      Specification
      Rabbit Reticulocyte
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 01 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Hamster Cell lysate - whole cell (BHK-21 (Kidney Fibroblasts))
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
      Loading amount
      500000 cells
      Specification
      BHK-21 (Kidney Fibroblasts)
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 31 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Dog Cell lysate - whole cell (Canine Kidney Epithelial MDCK cells)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
      Loading amount
      500000 cells
      Specification
      Canine Kidney Epithelial MDCK cells
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 21 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Human Foreskin Fibroblasts)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
      Loading amount
      200000 cells
      Specification
      Human Foreskin Fibroblasts
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 09 2016

      Western blot abreview for Anti-FADD (phospho S194) antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - whole cell (mouse embryonic fibroblasts (MEFs))
      Gel Running Conditions
      Reduced Denaturing (4-12% Bolt Bis-Tris Plus gradient gel)
      Loading amount
      200000 cells
      Specification
      mouse embryonic fibroblasts (MEFs)
      Blocking step
      Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 09 2016

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